Detailed information of TA system TA072484

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 2051888..2052650
• Replicon: chromosome
• Accession: NZ_CP019455
• Organism: Escherichia coli strain FHI_NMBU_03

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A0K5JE43
• Locus tag: BXO92_RS10365
• Protein ID: WP_028125934.1
• Coordinates: 2052360..2052650 (-)
• Length: 97 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: BXO92_RS10360
• Protein ID: WP_001534936.1
• Coordinates: 2051888..2052343 (-)
• Length: 152 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BXO92_RS10330	2047178..2048074	+	897	WP_000621626.1	sulfofructose kinase	-
BXO92_RS10335	2048108..2048893	+	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-
BXO92_RS10340	2048992..2049591	+	600	WP_001298594.1	glucose-1-phosphatase	-
BXO92_RS10345	2049585..2050457	+	873	WP_000920762.1	virulence factor BrkB family protein	-
BXO92_RS10350	2050454..2050891	+	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
BXO92_RS10355	2050936..2051877	+	942	WP_024223284.1	fatty acid biosynthesis protein FabY	-
BXO92_RS10360	2051888..2052343	-	456	WP_001534936.1	helix-turn-helix domain-containing protein	Antitoxin
BXO92_RS10365	2052360..2052650	-	291	WP_028125934.1	type II toxin-antitoxin system HigB family toxin	Toxin
BXO92_RS10375	2053049..2053267	+	219	WP_001314326.1	ribbon-helix-helix domain-containing protein	-
BXO92_RS10380	2053489..2054418	-	930	WP_104934070.1	formate dehydrogenase accessory protein FdhE	-
BXO92_RS10385	2054415..2055050	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
BXO92_RS10390	2055047..2055949	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (95-148)

Sequences

Toxin

Protein

• Length: 97 a.a.
• Molecular weight: 11608.45 Da
• Isoelectric Point: 8.8235

>T72484 WP_028125934.1 NZ_CP019455:c2052650-2052360 [Escherichia coli]
MHVISHKPFNEAIKRFPPYEGALIDLLNLLEKTEFSDPGEMKRYIPSLDNFKYRDKWWVINIAGNHLRLIAYIDFRLQKC
FIKHTVIYNEYDRFTT

Nucleotide

• Length: 291 bp

>T72484 NZ_CP019455:c2052650-2052360 [Escherichia coli]
ATGCATGTCATCAGCCACAAGCCTTTTAACGAGGCGATAAAACGCTTTCCACCTTACGAAGGTGCACTTATTGATTTATT
AAATCTTCTTGAGAAAACAGAGTTTAGCGATCCGGGTGAGATGAAGAGATATATTCCCTCACTGGATAATTTTAAATACA
GAGATAAATGGTGGGTTATTAATATTGCAGGTAATCATTTACGGCTTATCGCATATATTGATTTCAGGCTCCAAAAATGT
TTTATCAAACATACTGTTATCTACAATGAATACGACAGATTCACAACCTAG

Antitoxin

Protein

• Length: 152 a.a.
• Molecular weight: 17121.55 Da
• Isoelectric Point: 6.6011

>AT72484 WP_001534936.1 NZ_CP019455:c2052343-2051888 [Escherichia coli]
MRTHHTPAEDSANFGMLVKTFTHAVKDIPLCGPKADQEEYHNALKLIEFLVDRDDLENPLFELLCVRIREYENNAPEFQH
FNQRLASTPCGVSLLRILMDQHGLKAADLAQELGSKSNVSNILNGRRALTVRHIKALSERFNLPAEAFIDK

Nucleotide

• Length: 456 bp

>AT72484 NZ_CP019455:c2052343-2051888 [Escherichia coli]
ATGAGAACCCATCACACCCCAGCAGAAGACTCGGCCAATTTTGGAATGCTGGTTAAGACCTTCACCCACGCAGTGAAAGA
CATCCCTTTATGTGGCCCTAAAGCGGATCAAGAGGAATACCATAATGCGCTGAAACTGATCGAATTTTTAGTCGATCGTG
ACGATCTGGAAAACCCTTTATTTGAATTACTCTGCGTGCGGATCCGCGAATATGAAAATAACGCTCCAGAATTTCAGCAT
TTTAATCAGAGATTAGCATCGACCCCGTGCGGTGTAAGTCTGTTACGGATCCTTATGGATCAACATGGGCTTAAAGCGGC
TGATTTGGCACAAGAACTTGGCTCTAAATCTAATGTGAGTAATATTCTTAATGGTCGCCGGGCGCTCACCGTTCGCCATA
TCAAGGCGTTAAGCGAGCGTTTTAATCTCCCGGCTGAAGCGTTTATTGATAAGTAA

Structures

Toxin

• AlphaFold DB: A0A0K5JE43