Detailed information of TA system TA072478

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 1200776..1201575
• Replicon: chromosome
• Accession: NZ_CP019455
• Organism: Escherichia coli strain FHI_NMBU_03

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: S1NYM6
• Locus tag: BXO92_RS05985
• Protein ID: WP_000347251.1
• Coordinates: 1201111..1201575 (+)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: A0A0K4DCM8
• Locus tag: BXO92_RS05980
• Protein ID: WP_016233578.1
• Coordinates: 1200776..1201111 (+)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BXO92_RS05965	1196561..1197331	-	771	WP_001058227.1	2-dehydro-3-deoxyglucarate aldolase	-
BXO92_RS05970	1197347..1198681	-	1335	WP_000979025.1	galactarate/glucarate/glycerate transporter GarP	-
BXO92_RS05975	1199056..1200627	+	1572	WP_001273939.1	galactarate dehydratase	-
BXO92_RS05980	1200776..1201111	+	336	WP_016233578.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
BXO92_RS05985	1201111..1201575	+	465	WP_000347251.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
BXO92_RS05990	1201630..1202439	-	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
BXO92_RS06000	1202688..1203968	+	1281	WP_000681941.1	tagatose-bisphosphate aldolase subunit KbaZ	-
BXO92_RS06005	1203991..1204464	+	474	WP_001298322.1	PTS N-acetylgalactosamine transporter subunit IIB	-
BXO92_RS06010	1204475..1205254	+	780	WP_000406214.1	PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC	-
BXO92_RS06015	1205244..1206122	+	879	WP_001295548.1	PTS system mannose/fructose/sorbose family transporter subunit IID	-
BXO92_RS06020	1206140..1206574	+	435	WP_000948837.1	PTS sugar transporter subunit IIA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17777.14 Da
• Isoelectric Point: 9.4947

>T72478 WP_000347251.1 NZ_CP019455:1201111-1201575 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Nucleotide

• Length: 465 bp

>T72478 NZ_CP019455:1201111-1201575 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGATGCTTTAGTTGC
CGAAGTCGAGGCATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCTGCCACAAAGTTATTGGCGGTGGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGCTATCGTCTCTTCTTTCGTTACAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACGCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGTAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAAGCCTCACCCAAGAGACAGAAGAAAACCATTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12335.94 Da
• Isoelectric Point: 4.5392

>AT72478 WP_016233578.1 NZ_CP019455:1200776-1201111 [Escherichia coli]
MPANARSNAVLTTESKVTIRGQTTIPAPVREALKLKPGLDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFDIQQGKKLIAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT72478 NZ_CP019455:1200776-1201111 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTAACGCTGTACTGACCACTGAATCAAAGGTCACTATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCTGGCCTGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCGACATTCAACAAGGAAAGAAACTTATCGCTGGCATGGACGTCAACATTGACGATGAGAT
TGGCGACGACGAATAA

Structures

Toxin

• AlphaFold DB: A0A829CJ20

Antitoxin

• AlphaFold DB: A0A0K4DCM8