Detailed information of TA system TA072349

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3677957..3678651
• Replicon: chromosome
• Accession: NZ_CP019361
• Organism: Escherichia coli strain XH993

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1EXB8
• Locus tag: BW158_RS19825
• Protein ID: WP_001263493.1
• Coordinates: 3677957..3678355 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: BW158_RS19830
• Protein ID: WP_000554757.1
• Coordinates: 3678358..3678651 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BW158_RS19795	3672957..3674201	-	1245	WP_000189541.1	esterase FrsA	-
-	3673617..3673697	-	81	NuclAT_13	-	-
-	3673617..3673697	-	81	NuclAT_13	-	-
-	3673617..3673697	-	81	NuclAT_13	-	-
-	3673617..3673697	-	81	NuclAT_13	-	-
BW158_RS19800	3674293..3674751	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
BW158_RS19805	3675012..3676469	+	1458	WP_001293003.1	cytosol nonspecific dipeptidase	-
BW158_RS19810	3676526..3677047	-	522	Protein_3514	peptide chain release factor H	-
BW158_RS19815	3677046..3677249	-	204	Protein_3515	RNA ligase RtcB family protein	-
BW158_RS19820	3677495..3677947	-	453	WP_001059847.1	GNAT family N-acetyltransferase	-
BW158_RS19825	3677957..3678355	-	399	WP_001263493.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
BW158_RS19830	3678358..3678651	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
BW158_RS19835	3678703..3679758	-	1056	WP_001226164.1	DNA polymerase IV	-
BW158_RS19840	3679829..3680614	-	786	WP_000207564.1	putative lateral flagellar export/assembly protein LafU	-
BW158_RS19845	3680586..3682298	+	1713	Protein_3521	flagellar biosynthesis protein FlhA	-
BW158_RS19850	3682514..3683011	-	498	WP_000006260.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	gmhA/lpcA / tssA / hcp1/tssD1	3662999..3703675	40676

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15472.83 Da
• Isoelectric Point: 8.0949

>T72349 WP_001263493.1 NZ_CP019361:c3678355-3677957 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T72349 NZ_CP019361:c3678355-3677957 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACAGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT72349 WP_000554757.1 NZ_CP019361:c3678651-3678358 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT72349 NZ_CP019361:c3678651-3678358 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATTACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: S1EXB8

Antitoxin

• AlphaFold DB: S1FJN6