Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1821986..1822488 Replicon chromosome
Accession NZ_CP019361
Organism Escherichia coli strain XH993

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag BW158_RS10605 Protein ID WP_000767829.1
Coordinates 1822234..1822488 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag BW158_RS10600 Protein ID WP_001259255.1
Coordinates 1821986..1822237 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BW158_RS10575 1817164..1818231 - 1068 WP_113403919.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
BW158_RS10580 1818231..1819301 - 1071 WP_000108985.1 histidinol-phosphate transaminase -
BW158_RS10585 1819298..1820602 - 1305 WP_001300981.1 histidinol dehydrogenase -
BW158_RS10590 1820608..1821507 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
BW158_RS10595 1821653..1821703 - 51 WP_001364200.1 his operon leader peptide -
BW158_RS10600 1821986..1822237 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
BW158_RS10605 1822234..1822488 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
BW158_RS10610 1822571..1823395 + 825 WP_000754737.1 SDR family oxidoreductase -
BW158_RS10615 1823441..1824370 + 930 WP_000803374.1 LysR family transcriptional regulator -
BW158_RS10620 1824585..1824647 + 63 WP_010723108.1 membrane protein YoeI -
BW158_RS10625 1824637..1825995 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T72334 WP_000767829.1 NZ_CP019361:1822234-1822488 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T72334 NZ_CP019361:1822234-1822488 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT72334 WP_001259255.1 NZ_CP019361:1821986-1822237 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT72334 NZ_CP019361:1821986-1822237 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References