Detailed information of TA system TA072324

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 192591..192845
• Replicon: plasmid pXH993
• Accession: NZ_CP019360
• Organism: Escherichia coli strain XH993

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: -
• Locus tag: BW158_RS01040
• Protein ID: WP_001351576.1
• Coordinates: 192639..192845 (+)
• Length: 69 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	192591..192652 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BW158_RS01015	189339..190085	+	747	WP_000205749.1	conjugal transfer pilus acetylase TraX	-
BW158_RS01020	190144..191004	+	861	WP_000704513.1	alpha/beta hydrolase	-
BW158_RS01025	191107..191667	+	561	WP_000139315.1	fertility inhibition protein FinO	-
BW158_RS01030	191803..192015	+	213	WP_001299730.1	ANR family transcriptional regulator	-
BW158_RS01035	192261..192335	+	75	Protein_206	endonuclease	-
-	192591..192652	-	62	NuclAT_1	-	Antitoxin
-	192591..192652	-	62	NuclAT_1	-	Antitoxin
-	192591..192652	-	62	NuclAT_1	-	Antitoxin
-	192591..192652	-	62	NuclAT_1	-	Antitoxin
BW158_RS01040	192639..192845	+	207	WP_001351576.1	type I toxin-antitoxin system Hok family toxin	Toxin
BW158_RS01045	193129..193386	+	258	WP_000083845.1	replication regulatory protein RepA	-
BW158_RS01050	193618..193692	+	75	WP_001442103.1	RepA leader peptide Tap	-
BW158_RS01055	193685..194167	+	483	WP_001273588.1	hypothetical protein	-
BW158_RS01060	194160..195017	+	858	WP_168728579.1	incFII family plasmid replication initiator RepA	-
BW158_RS01065	195956..196609	+	654	WP_000616807.1	CPBP family intramembrane metalloprotease	-
BW158_RS01070	196702..196959	+	258	WP_000557619.1	type II toxin-antitoxin system antitoxin PemI	-
BW158_RS01075	196892..197293	+	402	WP_001398199.1	type II toxin-antitoxin system toxin endoribonuclease PemK	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	oqxB / oqxA / aac(3)-IId / sitABCD / mph(A) / aph(3')-Ia / sul2 / aph(4)-Ia / aac(3)-IVa / blaCTX-M-14 / fosA3 / mcr-1.1	iroN / iroE / iroD / iroC / iroB / iucA / iucB / iucC / iucD / iutA	1..349248	349248

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (23-64)

Sequences

Toxin

Protein

• Length: 69 a.a.
• Molecular weight: 7826.31 Da
• Isoelectric Point: 8.8807

>T72324 WP_001351576.1 NZ_CP019360:192639-192845 [Escherichia coli]
MKYLNTTDCSLFFAERSKFMTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 207 bp

>T72324 NZ_CP019360:192639-192845 [Escherichia coli]
ATGAAGTACCTTAACACTACTGATTGTAGCCTCTTCTTTGCTGAGAGGTCAAAGTTTATGACGAAATATGCCCTTATCGG
GTTGCTCGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTTATGTGAGCTGAATATTCACAGAG
GAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Nucleotide

• Length: 62 bp

>AT72324 NZ_CP019360:c192652-192591 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Antitoxin