Detailed information of TA system TA072319

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 171259..171529
• Replicon: plasmid pXH993
• Accession: NZ_CP019360
• Organism: Escherichia coli strain XH993

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: BW158_RS00910
• Protein ID: WP_001312861.1
• Coordinates: 171371..171529 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	171259..171322 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BW158_RS00870	166479..167450	+	972	WP_000817028.1	ParB/RepB/Spo0J family plasmid partition protein	-
BW158_RS00875	168589..168795	+	207	WP_000275856.1	hypothetical protein	-
BW158_RS00880	168821..169360	+	540	WP_000290840.1	single-stranded DNA-binding protein	-
BW158_RS00885	169428..169661	+	234	WP_000005990.1	DUF905 family protein	-
BW158_RS00890	169689..169886	+	198	Protein_177	hypothetical protein	-
BW158_RS00895	169941..170375	+	435	WP_000845936.1	conjugation system SOS inhibitor PsiB	-
BW158_RS00900	170372..171091	+	720	WP_001276238.1	plasmid SOS inhibition protein A	-
BW158_RS00905	171103..171291	-	189	WP_001299721.1	hypothetical protein	-
-	171103..171327	+	225	NuclAT_0	-	-
-	171103..171327	+	225	NuclAT_0	-	-
-	171103..171327	+	225	NuclAT_0	-	-
-	171103..171327	+	225	NuclAT_0	-	-
-	171259..171322	-	64	-	-	Antitoxin
BW158_RS00910	171371..171529	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
BW158_RS00915	172450..172737	+	288	WP_000107535.1	hypothetical protein	-
BW158_RS00920	172855..173676	+	822	WP_001234426.1	DUF945 domain-containing protein	-
BW158_RS00925	173973..174575	-	603	WP_000243712.1	transglycosylase SLT domain-containing protein	-
BW158_RS00930	174896..175279	+	384	WP_001151524.1	relaxosome protein TraM	-
BW158_RS00935	175466..176155	+	690	WP_000283385.1	conjugal transfer transcriptional regulator TraJ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	oqxB / oqxA / aac(3)-IId / sitABCD / mph(A) / aph(3')-Ia / sul2 / aph(4)-Ia / aac(3)-IVa / blaCTX-M-14 / fosA3 / mcr-1.1	iroN / iroE / iroD / iroC / iroB / iucA / iucB / iucC / iucD / iutA	1..349248	349248

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T72319 WP_001312861.1 NZ_CP019360:171371-171529 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T72319 NZ_CP019360:171371-171529 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 64 bp

>AT72319 NZ_CP019360:c171322-171259 [Escherichia coli]
GACTAGACATAGGGATGCCTCGTGGTGGTTAATGAAAATTAACTTACTACGGGGCTATCTTCTT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin