Detailed information of TA system TA072296

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 78739..79003
• Replicon: plasmid pXH990_3
• Accession: NZ_CP019358
• Organism: Escherichia coli strain XH990

Toxin (Protein)

• Gene name: pndA
• Uniprot ID: U9Z417
• Locus tag: BW242_RS02330
• Protein ID: WP_001387489.1
• Coordinates: 78739..78891 (-)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	pndB
Locus tag	-
Coordinates	78943..79003 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BW242_RS02300	74005..76173	+	2169	WP_015508354.1	DotA/TraY family protein	-
BW242_RS02305	76244..76906	+	663	WP_012783935.1	plasmid IncI1-type surface exclusion protein ExcA	-
BW242_RS02310	76978..77187	-	210	WP_000062603.1	HEAT repeat domain-containing protein	-
BW242_RS02315	77579..77755	+	177	WP_001054900.1	hypothetical protein	-
BW242_RS02320	77820..78116	-	297	WP_011264046.1	hypothetical protein	-
BW242_RS02325	78416..78667	+	252	Protein_91	hypothetical protein	-
BW242_RS02330	78739..78891	-	153	WP_001387489.1	Hok/Gef family protein	Toxin
-	78943..79003	+	61	NuclAT_0	-	Antitoxin
-	78943..79003	+	61	NuclAT_0	-	Antitoxin
-	78943..79003	+	61	NuclAT_0	-	Antitoxin
-	78943..79003	+	61	NuclAT_0	-	Antitoxin
BW242_RS02335	79206..80297	+	1092	WP_000426061.1	hypothetical protein	-
-	80367..80424	+	58	NuclAT_1	-	-
-	80367..80424	+	58	NuclAT_1	-	-
-	80367..80424	+	58	NuclAT_1	-	-
-	80367..80424	+	58	NuclAT_1	-	-
BW242_RS02340	80604..81812	+	1209	WP_001703845.1	IncI1-type conjugal transfer protein TrbA	-
BW242_RS02345	81831..82901	+	1071	WP_012783932.1	IncI1-type conjugal transfer protein TrbB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	blaCTX-M-55	-	1..86028	86028

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-46)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5761.10 Da
• Isoelectric Point: 8.7948

>T72296 WP_001387489.1 NZ_CP019358:c78891-78739 [Escherichia coli]
MPQRTFLMMLIVVCVTILCFVWMVRDSLCGLRLQQGNTVLVATLAYEVKR

Nucleotide

• Length: 153 bp

>T72296 NZ_CP019358:c78891-78739 [Escherichia coli]
ATGCCACAGCGAACGTTTTTAATGATGTTAATCGTCGTCTGTGTGACGATTCTGTGTTTTGTCTGGATGGTGAGGGATTC
GCTTTGCGGACTCCGGCTCCAGCAGGGAAACACAGTGCTTGTGGCAACGTTAGCCTACGAAGTTAAACGTTAA

Antitoxin

Nucleotide

• Length: 61 bp

>AT72296 NZ_CP019358:78943-79003 [Escherichia coli]
GAGGCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Structures

Toxin

• AlphaFold DB: U9Z417

Antitoxin