Detailed information of TA system TA072292

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 45331..45757
• Replicon: plasmid pXH990_2
• Accession: NZ_CP019357
• Organism: Escherichia coli strain XH990

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: BW242_RS01735
• Protein ID: WP_001312861.1
• Coordinates: 45331..45489 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	45533..45757 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BW242_RS01700	40354..40581	-	228	WP_000089263.1	conjugal transfer relaxosome protein TraY	-
BW242_RS01705	40718..41389	-	672	WP_000283561.1	conjugal transfer transcriptional regulator TraJ	-
BW242_RS01710	41583..41966	-	384	WP_001354030.1	relaxosome protein TraM	-
BW242_RS01715	42289..42891	+	603	WP_000243713.1	transglycosylase SLT domain-containing protein	-
BW242_RS01720	43188..44009	-	822	WP_001234445.1	DUF945 domain-containing protein	-
BW242_RS01725	44120..44416	-	297	WP_001272251.1	hypothetical protein	-
BW242_RS01730	44716..45093	+	378	Protein_55	hypothetical protein	-
BW242_RS01735	45331..45489	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	45533..45757	-	225	NuclAT_0	-	Antitoxin
-	45533..45757	-	225	NuclAT_0	-	Antitoxin
-	45533..45757	-	225	NuclAT_0	-	Antitoxin
-	45533..45757	-	225	NuclAT_0	-	Antitoxin
BW242_RS01740	45769..46488	-	720	WP_001276217.1	plasmid SOS inhibition protein A	-
BW242_RS01745	46485..46919	-	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
BW242_RS01750	46988..49011	-	2024	Protein_59	ParB/RepB/Spo0J family partition protein	-
BW242_RS01755	49072..49305	-	234	WP_000006003.1	DUF905 family protein	-
BW242_RS01760	49363..49890	-	528	WP_000290834.1	single-stranded DNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	erm(B)	-	1..67403	67403

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T72292 WP_001312861.1 NZ_CP019357:c45489-45331 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T72292 NZ_CP019357:c45489-45331 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 225 bp

>AT72292 NZ_CP019357:c45757-45533 [Escherichia coli]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin