Detailed information of TA system TA072252

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 2850153..2850735
• Replicon: chromosome
• Accession: NZ_CP019326
• Organism: Halomonas sp. 1513

Toxin (Protein)

• Gene name: graT
• Uniprot ID: -
• Locus tag: BWR19_RS12980
• Protein ID: WP_076748644.1
• Coordinates: 2850153..2850434 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: BWR19_RS12985
• Protein ID: WP_076748645.1
• Coordinates: 2850445..2850735 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BWR19_RS19240	2845648..2846379	-	732	WP_156889349.1	hypothetical protein	-
BWR19_RS12960	2846573..2847001	-	429	WP_076748641.1	fosfomycin resistance glutathione transferase	-
BWR19_RS12965	2847307..2848113	-	807	WP_076748642.1	indole-3-glycerol phosphate synthase TrpC	-
BWR19_RS12970	2848150..2849169	-	1020	WP_076748643.1	anthranilate phosphoribosyltransferase	-
BWR19_RS12975	2849183..2849770	-	588	WP_076750105.1	aminodeoxychorismate/anthranilate synthase component II	-
BWR19_RS12980	2850153..2850434	+	282	WP_076748644.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BWR19_RS12985	2850445..2850735	+	291	WP_076748645.1	HigA family addiction module antidote protein	Antitoxin
BWR19_RS12990	2850830..2851078	+	249	Protein_2560	nucleotidyl transferase AbiEii/AbiGii toxin family protein	-
BWR19_RS12995	2851242..2852264	-	1023	WP_076748646.1	hypothetical protein	-
BWR19_RS13000	2852257..2852766	-	510	WP_076748647.1	hypothetical protein	-
BWR19_RS13005	2852824..2853822	-	999	WP_076748648.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	2835865..2856429	20564

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (16-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (23-70)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10782.33 Da
• Isoelectric Point: 9.9563

>T72252 WP_076748644.1 NZ_CP019326:2850153-2850434 [Halomonas sp. 1513]
MIKSVKDKETASIIRGKLSRKLPHDMQRSALKRLRQLDAAVSLDDLRIPPGNRLEALQGDRGGQYSIRINDQWRLCFRFQ
EGNAYDVEIVDYH

Nucleotide

• Length: 282 bp

>T72252 NZ_CP019326:2850153-2850434 [Halomonas sp. 1513]
ATGATCAAAAGTGTCAAGGATAAAGAGACGGCCAGCATTATACGCGGCAAGCTCTCCCGAAAATTGCCGCATGATATGCA
GCGAAGCGCGCTCAAGAGACTTCGCCAACTGGATGCAGCCGTCAGCCTTGATGACCTTCGCATTCCACCTGGAAACCGCC
TCGAGGCCCTTCAAGGTGATCGCGGAGGACAATACAGCATACGAATAAACGATCAGTGGCGACTTTGCTTCCGCTTTCAA
GAAGGCAACGCCTATGACGTGGAAATCGTCGACTACCACTGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10811.26 Da
• Isoelectric Point: 7.2015

>AT72252 WP_076748645.1 NZ_CP019326:2850445-2850735 [Halomonas sp. 1513]
MKTQQLPNIHPGEILLEDFIEPMGLTKNALANAISVPATRIGEITRGARAITADTDLRLSRYFGTSEGYWLRLQNAYDLE
QARRAGRYEEIRPHAA

Nucleotide

• Length: 291 bp

>AT72252 NZ_CP019326:2850445-2850735 [Halomonas sp. 1513]
ATGAAGACTCAGCAACTACCCAACATACATCCTGGTGAGATCCTGCTAGAGGATTTTATCGAGCCTATGGGGCTCACCAA
GAATGCGCTAGCCAACGCTATCAGCGTACCGGCTACACGGATCGGTGAGATTACCCGCGGCGCACGAGCCATTACCGCCG
ACACCGATCTTCGCCTGTCGCGCTACTTCGGCACCAGCGAAGGTTACTGGCTGCGCCTGCAAAACGCCTATGATCTTGAG
CAAGCACGTCGGGCTGGGCGCTACGAAGAGATACGCCCCCACGCCGCCTGA