Detailed information of TA system TA072210

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 110638..110908
• Replicon: plasmid p13P484A-2
• Accession: NZ_CP019282
• Organism: Escherichia coli strain 13P484A

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: BWI90_RS25815
• Protein ID: WP_001312861.1
• Coordinates: 110750..110908 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	110638..110701 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BWI90_RS25775	105858..106829	+	972	WP_000817028.1	ParB/RepB/Spo0J family plasmid partition protein	-
BWI90_RS27960	107968..108174	+	207	WP_000275856.1	hypothetical protein	-
BWI90_RS25790	108200..108739	+	540	WP_000290840.1	single-stranded DNA-binding protein	-
BWI90_RS25795	108807..109040	+	234	WP_000005987.1	DUF905 family protein	-
BWI90_RS25800	109068..109265	+	198	Protein_113	hypothetical protein	-
BWI90_RS25805	109320..109754	+	435	WP_000845936.1	conjugation system SOS inhibitor PsiB	-
BWI90_RS25810	109751..110470	+	720	WP_001276238.1	plasmid SOS inhibition protein A	-
BWI90_RS27730	110482..110670	-	189	WP_001299721.1	hypothetical protein	-
-	110482..110706	+	225	NuclAT_0	-	-
-	110482..110706	+	225	NuclAT_0	-	-
-	110482..110706	+	225	NuclAT_0	-	-
-	110482..110706	+	225	NuclAT_0	-	-
-	110638..110701	-	64	-	-	Antitoxin
BWI90_RS25815	110750..110908	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
BWI90_RS25830	111829..112116	+	288	WP_000107535.1	hypothetical protein	-
BWI90_RS25835	112234..113055	+	822	Protein_119	DUF932 domain-containing protein	-
BWI90_RS25840	113352..113954	-	603	WP_000243712.1	transglycosylase SLT domain-containing protein	-
BWI90_RS25845	114275..114658	+	384	WP_001151524.1	relaxosome protein TraM	-
BWI90_RS25850	114845..115534	+	690	WP_000283385.1	conjugal transfer transcriptional regulator TraJ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaTEM-1B / blaCTX-M-15 / fosA3 / mph(A) / qacE / aadA2 / dfrA12 / oqxB / oqxA / tet(A) / sitABCD	iroN / iroE / iroD / iroC / iroB / iucA / iucB / iucC / iucD / iutA	1..149520	149520

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T72210 WP_001312861.1 NZ_CP019282:110750-110908 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T72210 NZ_CP019282:110750-110908 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 64 bp

>AT72210 NZ_CP019282:c110701-110638 [Escherichia coli]
GACTAGACATAGGGATGCCTCGTGGTGGTTAATGAAAATTAACTTACTACGGGGCTATCTTCTT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin