Detailed information of TA system TA072205

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mqsRA (relBE)/MqsR-MqsA
• Location: 3302034..3302727
• Replicon: chromosome
• Accession: NZ_CP019280
• Organism: Escherichia coli strain 13P484A

Toxin (Protein)

• Gene name: mqsR
• Uniprot ID: S1EZG2
• Locus tag: BWI90_RS17355
• Protein ID: WP_000415584.1
• Coordinates: 3302431..3302727 (-)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: mqsA
• Uniprot ID: -
• Locus tag: BWI90_RS17350
• Protein ID: WP_148119789.1
• Coordinates: 3302034..3302429 (-)
• Length: 132 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BWI90_RS17335	3297898..3300156	-	2259	WP_001281841.1	DNA topoisomerase IV subunit A	-
BWI90_RS17340	3300294..3301901	-	1608	WP_001295629.1	ABC transporter substrate-binding protein	-
BWI90_RS17350	3302034..3302429	-	396	WP_148119789.1	type II toxin-antitoxin system antitoxin MqsA	Antitoxin
BWI90_RS17355	3302431..3302727	-	297	WP_000415584.1	type II toxin-antitoxin system toxin MqsR	Toxin
BWI90_RS17360	3302932..3303414	-	483	WP_000183505.1	transcriptional regulator	-
BWI90_RS17365	3303467..3303859	-	393	WP_000712658.1	OB fold stress tolerance protein YgiW	-
BWI90_RS17370	3304011..3304670	+	660	WP_001221493.1	two-component system response regulator QseB	-
BWI90_RS17375	3304667..3306016	+	1350	WP_000673402.1	two-component system sensor histidine kinase QseC	-
BWI90_RS17380	3306062..3306395	-	334	Protein_3146	DUF2645 family protein	-
BWI90_RS17390	3306714..3307295	+	582	WP_000065430.1	NADPH:quinone oxidoreductase MdaB	-
BWI90_RS17395	3307326..3307640	+	315	WP_000958598.1	antibiotic biosynthesis monooxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin MqsR (5-97)

Antitoxin

• Zinc finger, MqsA-type (3-47)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11231.96 Da
• Isoelectric Point: 8.9070

>T72205 WP_000415584.1 NZ_CP019280:c3302727-3302431 [Escherichia coli]
MEKRTPHTRLSQVKKLVNAGQVRTTRSALLNADELGLDFDGMCNVIIGLSESDFYKSMTTYSDHTIWQDVYRPRLVTGQV
YLKITVIHDVLIVSFKEK

Nucleotide

• Length: 297 bp

>T72205 NZ_CP019280:c3302727-3302431 [Escherichia coli]
ATGGAAAAACGCACACCACATACACGTTTGAGTCAGGTTAAAAAACTTGTCAATGCCGGGCAAGTTCGTACAACACGTAG
TGCACTGTTAAATGCAGATGAGTTAGGTTTGGATTTTGATGGTATGTGTAATGTTATCATTGGATTATCAGAGAGCGACT
TTTATAAAAGCATGACCACCTACTCTGATCATACTATCTGGCAGGATGTTTACAGACCCAGGCTTGTTACAGGCCAGGTT
TATCTTAAAATTACGGTAATTCATGACGTACTGATCGTCTCGTTTAAGGAGAAGTAA

Antitoxin

Protein

• Length: 132 a.a.
• Molecular weight: 14731.15 Da
• Isoelectric Point: 9.2136

>AT72205 WP_148119789.1 NZ_CP019280:c3302429-3302034 [Escherichia coli]
MKCPVCHQGEMVSGIKDIPYTFRGRKTVLKGIHGLYCVHCEESIMNKEESDAFMAQVKAFRASVNVETVAPEFIVKVRKK
LSLTQKEASEIFGGGVNAFSRYEKGNAQPHPSTIKLLRVLDKHPELLNEIR

Nucleotide

• Length: 396 bp

>AT72205 NZ_CP019280:c3302429-3302034 [Escherichia coli]
ATGAAATGTCCGGTTTGCCACCAGGGAGAAATGGTTTCTGGCATTAAAGATATTCCATACACCTTCCGTGGACGAAAAAC
AGTATTGAAAGGTATCCACGGTTTATATTGTGTCCATTGCGAAGAGAGCATCATGAATAAAGAAGAGTCAGATGCTTTCA
TGGCGCAAGTAAAGGCATTTCGGGCTTCGGTGAATGTCGAAACAGTGGCACCTGAATTTATAGTGAAGGTTCGAAAAAAG
CTCTCTCTTACCCAAAAAGAGGCAAGCGAAATTTTTGGGGGAGGTGTAAATGCGTTTTCGCGTTACGAAAAAGGCAATGC
CCAACCTCATCCTTCCACAATCAAACTTTTACGTGTTCTGGATAAGCATCCAGAACTATTGAATGAAATCCGTTAA

Structures

Toxin

• PDB: 3HI2