Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2218684..2219186 Replicon chromosome
Accession NZ_CP019280
Organism Escherichia coli strain 13P484A

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag BWI90_RS11885 Protein ID WP_000767829.1
Coordinates 2218684..2218938 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag BWI90_RS11890 Protein ID WP_001259255.1
Coordinates 2218935..2219186 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BWI90_RS11855 2213699..2213926 - 228 WP_000985273.1 sulfurtransferase TusA family protein -
BWI90_RS11860 2213940..2214998 - 1059 WP_000492330.1 transport protein YeeE -
BWI90_RS11865 2215177..2216535 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
BWI90_RS11870 2216525..2216587 - 63 WP_010723108.1 membrane protein YoeI -
BWI90_RS11875 2216802..2217731 - 930 WP_000803366.1 LysR family transcriptional regulator -
BWI90_RS11880 2217777..2218601 - 825 WP_000754737.1 SDR family oxidoreductase -
BWI90_RS11885 2218684..2218938 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
BWI90_RS11890 2218935..2219186 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
BWI90_RS11895 2219470..2219520 + 51 WP_001364200.1 his operon leader peptide -
BWI90_RS11905 2219666..2220565 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
BWI90_RS11910 2220571..2221875 + 1305 WP_001446927.1 histidinol dehydrogenase -
BWI90_RS11915 2221872..2222942 + 1071 WP_000108933.1 histidinol-phosphate transaminase -
BWI90_RS11920 2222942..2224009 + 1068 WP_000080090.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T72201 WP_000767829.1 NZ_CP019280:c2218938-2218684 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T72201 NZ_CP019280:c2218938-2218684 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT72201 WP_001259255.1 NZ_CP019280:c2219186-2218935 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT72201 NZ_CP019280:c2219186-2218935 [Escherichia coli]
ATGCGTACAATTAGTTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References