Detailed information of TA system TA072182

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 301430..302124
• Replicon: chromosome
• Accession: NZ_CP019280
• Organism: Escherichia coli strain 13P484A

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: -
• Locus tag: BWI90_RS01460
• Protein ID: WP_089578406.1
• Coordinates: 301726..302124 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: BWI90_RS01455
• Protein ID: WP_000554757.1
• Coordinates: 301430..301723 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BWI90_RS01435	297070..297567	+	498	WP_000006261.1	REP-associated tyrosine transposase RayT	-
BWI90_RS01440	297783..299495	-	1713	Protein_269	flagellar biosynthesis protein FlhA	-
BWI90_RS01445	299467..300252	+	786	WP_072132640.1	putative lateral flagellar export/assembly protein LafU	-
BWI90_RS01450	300323..301378	+	1056	WP_148119675.1	DNA polymerase IV	-
BWI90_RS01455	301430..301723	+	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
BWI90_RS01460	301726..302124	+	399	WP_089578406.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
BWI90_RS01465	302134..302586	+	453	WP_001059847.1	GNAT family N-acetyltransferase	-
BWI90_RS01470	302832..303035	+	204	Protein_275	RNA ligase RtcB family protein	-
BWI90_RS01475	303034..303555	+	522	Protein_276	peptide chain release factor H	-
BWI90_RS01480	303612..305069	-	1458	WP_001293003.1	cytosol nonspecific dipeptidase	-
BWI90_RS01485	305330..305788	+	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
BWI90_RS01490	305880..307124	+	1245	WP_000189541.1	esterase FrsA	-
-	306384..306464	+	81	NuclAT_12	-	-
-	306384..306464	+	81	NuclAT_12	-	-
-	306384..306464	+	81	NuclAT_12	-	-
-	306384..306464	+	81	NuclAT_12	-	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15506.85 Da
• Isoelectric Point: 8.0949

>T72182 WP_089578406.1 NZ_CP019280:301726-302124 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHFVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T72182 NZ_CP019280:301726-302124 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTTGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACAGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT72182 WP_000554757.1 NZ_CP019280:301430-301723 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT72182 NZ_CP019280:301430-301723 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATTACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Antitoxin

• AlphaFold DB: S1FJN6