Detailed information of TA system TA072172

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 5598..6024
• Replicon: plasmid p13P477T-3
• Accession: NZ_CP019276
• Organism: Escherichia coli strain 13P477T

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: BWI89_RS26730
• Protein ID: WP_001312861.1
• Coordinates: 5598..5756 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	5800..6024 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BWI89_RS26680	654..881	-	228	WP_001254386.1	conjugal transfer relaxosome protein TraY	-
BWI89_RS26685	1092..1664	-	573	WP_199777250.1	PAS domain-containing protein	-
BWI89_RS26695	1855..2236	-	382	Protein_4	relaxosome protein TraM	-
BWI89_RS26700	2514..3160	+	647	Protein_5	transglycosylase SLT domain-containing protein	-
BWI89_RS26705	3456..4277	-	822	WP_001234445.1	DUF945 domain-containing protein	-
BWI89_RS26710	4387..4683	-	297	WP_001272251.1	hypothetical protein	-
BWI89_RS29685	4983..5360	+	378	Protein_8	hypothetical protein	-
BWI89_RS26730	5598..5756	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	5800..6024	-	225	NuclAT_0	-	Antitoxin
-	5800..6024	-	225	NuclAT_0	-	Antitoxin
-	5800..6024	-	225	NuclAT_0	-	Antitoxin
-	5800..6024	-	225	NuclAT_0	-	Antitoxin
BWI89_RS29390	5836..6015	+	180	WP_001309233.1	hypothetical protein	-
BWI89_RS26735	6036..6755	-	720	WP_001276217.1	plasmid SOS inhibition protein A	-
BWI89_RS26740	6752..7186	-	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
BWI89_RS26745	7255..9278	-	2024	Protein_13	ParB/RepB/Spo0J family partition protein	-
BWI89_RS26750	9339..9572	-	234	WP_000006003.1	DUF905 family protein	-
BWI89_RS26755	9630..10157	-	528	WP_000290834.1	single-stranded DNA-binding protein	-
BWI89_RS29690	10459..10926	+	468	WP_010891336.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaCTX-M-55 / blaTEM-1B / fosA3	-	1..91701	91701

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T72172 WP_001312861.1 NZ_CP019276:c5756-5598 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T72172 NZ_CP019276:c5756-5598 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 225 bp

>AT72172 NZ_CP019276:c6024-5800 [Escherichia coli]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin