Detailed information of TA system TA072169

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 4568442..4569099
• Replicon: chromosome
• Accession: NZ_CP019273
• Organism: Escherichia coli strain 13P477T

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U3PDC3
• Locus tag: BWI89_RS23835
• Protein ID: WP_000270043.1
• Coordinates: 4568749..4569099 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: BWI89_RS23830
• Protein ID: WP_000124640.1
• Coordinates: 4568442..4568744 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BWI89_RS23775	4563593..4564033	+	441	Protein_4334	DsbA family protein	-
BWI89_RS23780	4564067..4564495	+	429	WP_000591074.1	hypothetical protein	-
BWI89_RS23785	4564552..4564911	+	360	WP_000422768.1	hypothetical protein	-
BWI89_RS23790	4564911..4565357	+	447	WP_000919345.1	Fe3+-siderophore ABC transporter permease	-
BWI89_RS23795	4565354..4565872	+	519	WP_000210756.1	nitrite reductase	-
BWI89_RS23800	4565872..4566102	+	231	WP_000972663.1	hypothetical protein	-
BWI89_RS23805	4566089..4566946	+	858	WP_001167032.1	hypothetical protein	-
BWI89_RS23815	4567177..4567704	+	528	WP_001236377.1	thermonuclease family protein	-
BWI89_RS23820	4567762..4568034	+	273	WP_001043047.1	HU family DNA-binding protein	-
BWI89_RS23825	4568122..4568415	+	294	WP_001239997.1	chromosome segregation protein ParM	-
BWI89_RS23830	4568442..4568744	-	303	WP_000124640.1	XRE family transcriptional regulator	Antitoxin
BWI89_RS23835	4568749..4569099	-	351	WP_000270043.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BWI89_RS23840	4569262..4569810	+	549	WP_001061574.1	transcriptional regulator	-
BWI89_RS23850	4570151..4570345	+	195	WP_000343597.1	hypothetical protein	-
BWI89_RS23855	4570356..4570727	+	372	WP_000516916.1	hypothetical protein	-
BWI89_RS23860	4570720..4571190	+	471	WP_001281821.1	hypothetical protein	-
BWI89_RS23865	4571205..4571540	-	336	WP_000683476.1	hypothetical protein	-
BWI89_RS23870	4571637..4572125	+	489	WP_001273096.1	DUF1643 domain-containing protein	-
BWI89_RS23875	4572128..4572625	+	498	WP_000062185.1	hypothetical protein	-
BWI89_RS23880	4572840..4573544	-	705	WP_001067855.1	IS6-like element IS26 family transposase	-
BWI89_RS23885	4573609..4573788	-	180	Protein_4354	winged helix-turn-helix domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	floR / tet(A) / aph(6)-Id / aph(3'')-Ib / sul2 / blaCMY-2	rfaE / gspM / gspL / gspK / gspJ / gspI / gspH / gspG / gspF / gspE / gspD / gspC	4429227..4598400	169173

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-79)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13328.21 Da
• Isoelectric Point: 5.2421

>T72169 WP_000270043.1 NZ_CP019273:c4569099-4568749 [Escherichia coli]
MWVIETTDTFDEWFDALDDTDRANVLASMMVLRDRGPMLSRPYADTVNGSSYSNMKELRVQSKGDPIRAFFAFDPKRKGI
LLCAGNKTGDEKRFYEVMIPIADREFAAHLDKLKKE

Nucleotide

• Length: 351 bp

>T72169 NZ_CP019273:c4569099-4568749 [Escherichia coli]
ATGTGGGTCATCGAGACAACCGACACCTTTGATGAGTGGTTCGATGCTCTGGATGATACCGATAGAGCAAACGTGCTGGC
TTCGATGATGGTGCTGCGAGATAGAGGCCCCATGCTGTCGAGGCCATACGCGGATACTGTTAACGGTTCATCCTACAGCA
ACATGAAAGAGCTTCGGGTCCAAAGCAAAGGAGATCCTATCAGAGCGTTCTTTGCGTTCGATCCAAAGCGTAAGGGGATT
CTTCTCTGCGCCGGTAACAAGACCGGGGACGAGAAAAGGTTTTATGAAGTAATGATCCCAATTGCAGACCGTGAGTTTGC
GGCGCACTTGGATAAATTGAAGAAGGAGTGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11006.73 Da
• Isoelectric Point: 7.2036

>AT72169 WP_000124640.1 NZ_CP019273:c4568744-4568442 [Escherichia coli]
MARTLDQMLATEKPEVVAKAQKAATEMLLNIHLAELRDRMNLTQGEIAASLGVRQPTVSEMEKPGRDLKLSSIKRYVEAS
GGKLRLDVELPDGTHYGFAV

Nucleotide

• Length: 303 bp

>AT72169 NZ_CP019273:c4568744-4568442 [Escherichia coli]
ATGGCAAGAACTCTTGACCAAATGCTGGCAACAGAAAAGCCTGAGGTTGTTGCCAAAGCACAGAAAGCGGCCACTGAGAT
GTTACTGAACATCCACTTGGCAGAGCTTCGTGATCGCATGAACCTTACCCAGGGGGAAATTGCTGCATCTCTGGGTGTGA
GACAGCCGACAGTCTCCGAGATGGAAAAACCTGGACGAGATCTCAAGCTGTCGTCCATTAAGCGGTACGTTGAGGCCTCC
GGTGGCAAGCTACGCCTCGATGTTGAACTTCCGGACGGCACCCACTACGGCTTTGCCGTTTAA

Structures

Toxin

• AlphaFold DB: A0A7G8AFB9