Detailed information of TA system TA072161

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 3486951..3487553
• Replicon: chromosome
• Accession: NZ_CP019273
• Organism: Escherichia coli strain 13P477T

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: BWI89_RS18140
• Protein ID: WP_000897305.1
• Coordinates: 3487242..3487553 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: BWI89_RS18135
• Protein ID: WP_000356397.1
• Coordinates: 3486951..3487241 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BWI89_RS18115	3483453..3484355	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
BWI89_RS18120	3484352..3484987	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
BWI89_RS18125	3484984..3485913	+	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
BWI89_RS18130	3486128..3486346	-	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
BWI89_RS18135	3486951..3487241	-	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
BWI89_RS18140	3487242..3487553	-	312	WP_000897305.1	hypothetical protein	Toxin
BWI89_RS18145	3487782..3488690	+	909	WP_001318161.1	alpha/beta hydrolase	-
BWI89_RS18150	3488754..3489695	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
BWI89_RS18155	3489740..3490177	-	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
BWI89_RS18160	3490174..3491046	-	873	WP_000920762.1	virulence factor BrkB family protein	-
BWI89_RS18165	3491040..3491639	-	600	WP_001315111.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T72161 WP_000897305.1 NZ_CP019273:c3487553-3487242 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T72161 NZ_CP019273:c3487553-3487242 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT72161 WP_000356397.1 NZ_CP019273:c3487241-3486951 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT72161 NZ_CP019273:c3487241-3486951 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7