Detailed information of TA system TA072139

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 126330..126583
• Replicon: plasmid p13P460A-1
• Accession: NZ_CP019272
• Organism: Escherichia coli strain 13P460A

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: BWI88_RS25330
• Protein ID: WP_001312851.1
• Coordinates: 126434..126583 (+)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	126330..126389 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BWI88_RS25300	123057..123803	+	747	WP_000205770.1	conjugal transfer pilus acetylase TraX	-
BWI88_RS25305	123858..124418	+	561	WP_000139328.1	fertility inhibition protein FinO	-
BWI88_RS25310	124550..124750	+	201	WP_015059022.1	hypothetical protein	-
BWI88_RS25315	125136..125735	+	600	WP_032083981.1	hypothetical protein	-
-	126330..126389	-	60	NuclAT_1	-	Antitoxin
-	126330..126389	-	60	NuclAT_1	-	Antitoxin
-	126330..126389	-	60	NuclAT_1	-	Antitoxin
-	126330..126389	-	60	NuclAT_1	-	Antitoxin
BWI88_RS25330	126434..126583	+	150	WP_001312851.1	type I toxin-antitoxin system Hok family toxin	Toxin
BWI88_RS25335	126870..127616	-	747	WP_001016257.1	IS21-like element ISEc12 family helper ATPase IstB	-
BWI88_RS25340	127631..129172	-	1542	WP_001298859.1	IS21-like element ISEc12 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	floR / tet(A) / aph(6)-Id / aph(3'')-Ib / sul2 / oqxA / oqxB / aph(3')-IIa / fosA3 / blaTEM-1B / blaCTX-M-55	-	1..129452	129452

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T72139 WP_001312851.1 NZ_CP019272:126434-126583 [Escherichia coli]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T72139 NZ_CP019272:126434-126583 [Escherichia coli]
ATGACGAAATATGCCCTTATCGGGTTGCTCGCCGTGTGTGCCACGGTATTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAACTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Nucleotide

• Length: 60 bp

>AT72139 NZ_CP019272:c126389-126330 [Escherichia coli]
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin