Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 3614758..3615260 Replicon chromosome
Accession NZ_CP019271
Organism Escherichia coli strain 13P460A

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag BWI88_RS18320 Protein ID WP_000767829.1
Coordinates 3615006..3615260 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag BWI88_RS18315 Protein ID WP_001259255.1
Coordinates 3614758..3615009 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BWI88_RS18285 3609935..3611002 - 1068 WP_000080093.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
BWI88_RS18290 3611002..3612072 - 1071 WP_148125482.1 histidinol-phosphate transaminase -
BWI88_RS18295 3612069..3613373 - 1305 WP_001446927.1 histidinol dehydrogenase -
BWI88_RS18300 3613379..3614278 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
BWI88_RS18310 3614424..3614474 - 51 WP_001364200.1 his operon leader peptide -
BWI88_RS18315 3614758..3615009 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
BWI88_RS18320 3615006..3615260 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
BWI88_RS18325 3615343..3616167 + 825 WP_000754737.1 SDR family oxidoreductase -
BWI88_RS18330 3616213..3617142 + 930 WP_000803366.1 LysR family transcriptional regulator -
BWI88_RS18335 3617357..3617419 + 63 WP_010723108.1 membrane protein YoeI -
BWI88_RS18340 3617409..3618767 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
BWI88_RS18345 3618946..3620004 + 1059 WP_000492330.1 transport protein YeeE -
BWI88_RS18350 3620018..3620245 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T72120 WP_000767829.1 NZ_CP019271:3615006-3615260 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T72120 NZ_CP019271:3615006-3615260 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT72120 WP_001259255.1 NZ_CP019271:3614758-3615009 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT72120 NZ_CP019271:3614758-3615009 [Escherichia coli]
ATGCGTACAATTAGTTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References