Detailed information of TA system TA072116

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 2439983..2440782
• Replicon: chromosome
• Accession: NZ_CP019271
• Organism: Escherichia coli strain 13P460A

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: F4VJD3
• Locus tag: BWI88_RS12400
• Protein ID: WP_000347266.1
• Coordinates: 2439983..2440447 (-)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: S1EB98
• Locus tag: BWI88_RS12405
• Protein ID: WP_001307405.1
• Coordinates: 2440447..2440782 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BWI88_RS12365	2434984..2435418	-	435	WP_000948824.1	PTS sugar transporter subunit IIA	-
BWI88_RS12370	2435436..2436314	-	879	WP_001315856.1	PTS system mannose/fructose/sorbose family transporter subunit IID	-
BWI88_RS12375	2436304..2437083	-	780	WP_000406214.1	PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC	-
BWI88_RS12380	2437094..2437567	-	474	WP_001295547.1	PTS N-acetylgalactosamine transporter subunit IIB	-
BWI88_RS12385	2437590..2438870	-	1281	WP_000681921.1	tagatose-bisphosphate aldolase subunit KbaZ	-
BWI88_RS12395	2439119..2439928	+	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
BWI88_RS12400	2439983..2440447	-	465	WP_000347266.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
BWI88_RS12405	2440447..2440782	-	336	WP_001307405.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
BWI88_RS12410	2440931..2442502	-	1572	WP_001273741.1	galactarate dehydratase	-
BWI88_RS12415	2442877..2444211	+	1335	WP_000599636.1	galactarate/glucarate/glycerate transporter GarP	-
BWI88_RS12420	2444227..2444997	+	771	WP_001058227.1	2-dehydro-3-deoxyglucarate aldolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	2439983..2451770	11787

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17841.18 Da
• Isoelectric Point: 9.4947

>T72116 WP_000347266.1 NZ_CP019271:c2440447-2439983 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRFFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Nucleotide

• Length: 465 bp

>T72116 NZ_CP019271:c2440447-2439983 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTTTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAAGCCTCACCCAAGAGACAGAAGAAAACCATTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12358.94 Da
• Isoelectric Point: 4.8616

>AT72116 WP_001307405.1 NZ_CP019271:c2440782-2440447 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT72116 NZ_CP019271:c2440782-2440447 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTCTTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Structures

Toxin

• AlphaFold DB: A0A836NGD2

Antitoxin

• AlphaFold DB: A0A0E0XVC7