Detailed information of TA system TA072114

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 1624775..1625377
• Replicon: chromosome
• Accession: NZ_CP019271
• Organism: Escherichia coli strain 13P460A

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: BWI88_RS08175
• Protein ID: WP_000897305.1
• Coordinates: 1625066..1625377 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: BWI88_RS08170
• Protein ID: WP_000356397.1
• Coordinates: 1624775..1625065 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BWI88_RS08140	1620700..1621602	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
BWI88_RS08145	1621599..1622234	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
BWI88_RS08150	1622231..1623160	+	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
BWI88_RS08155	1623490..1623732	-	243	WP_001087409.1	hypothetical protein	-
BWI88_RS08160	1623952..1624170	-	219	WP_001315930.1	ribbon-helix-helix domain-containing protein	-
BWI88_RS08170	1624775..1625065	-	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
BWI88_RS08175	1625066..1625377	-	312	WP_000897305.1	hypothetical protein	Toxin
BWI88_RS08180	1625606..1626514	+	909	WP_001162704.1	alpha/beta hydrolase	-
BWI88_RS08185	1626578..1627519	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
BWI88_RS08190	1627564..1628001	-	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
BWI88_RS08195	1627998..1628870	-	873	WP_000920762.1	virulence factor BrkB family protein	-
BWI88_RS08200	1628864..1629463	-	600	WP_001295269.1	glucose-1-phosphatase	-
BWI88_RS08205	1629562..1630347	-	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T72114 WP_000897305.1 NZ_CP019271:c1625377-1625066 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T72114 NZ_CP019271:c1625377-1625066 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTTTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTATTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT72114 WP_000356397.1 NZ_CP019271:c1625065-1624775 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT72114 NZ_CP019271:c1625065-1624775 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7