Detailed information of TA system TA072096

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 114109..114379
• Replicon: plasmid p13C1079T-1
• Accession: NZ_CP019268
• Organism: Escherichia coli strain 13C1079T

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: BWI87_RS25155
• Protein ID: WP_001312861.1
• Coordinates: 114221..114379 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	114109..114172 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BWI87_RS25115	109329..110300	+	972	WP_000817028.1	ParB/RepB/Spo0J family plasmid partition protein	-
BWI87_RS26195	111439..111645	+	207	WP_000275856.1	hypothetical protein	-
BWI87_RS25130	111671..112210	+	540	WP_000290840.1	single-stranded DNA-binding protein	-
BWI87_RS25135	112278..112511	+	234	WP_000005987.1	DUF905 family protein	-
BWI87_RS25140	112539..112736	+	198	Protein_112	hypothetical protein	-
BWI87_RS25145	112791..113225	+	435	WP_000845936.1	conjugation system SOS inhibitor PsiB	-
BWI87_RS25150	113222..113941	+	720	WP_001276238.1	plasmid SOS inhibition protein A	-
BWI87_RS26040	113953..114141	-	189	WP_001299721.1	hypothetical protein	-
-	113953..114177	+	225	NuclAT_0	-	-
-	113953..114177	+	225	NuclAT_0	-	-
-	113953..114177	+	225	NuclAT_0	-	-
-	113953..114177	+	225	NuclAT_0	-	-
-	114109..114172	-	64	-	-	Antitoxin
BWI87_RS25155	114221..114379	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
BWI87_RS25170	115300..115587	+	288	WP_000107535.1	hypothetical protein	-
BWI87_RS25175	115705..116526	+	822	WP_001234426.1	DUF945 domain-containing protein	-
BWI87_RS25180	116824..117426	-	603	WP_000243712.1	transglycosylase SLT domain-containing protein	-
BWI87_RS25185	117747..118130	+	384	WP_001151524.1	relaxosome protein TraM	-
BWI87_RS25190	118317..119006	+	690	WP_000283385.1	conjugal transfer transcriptional regulator TraJ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	aph(3')-Ia / aph(6)-Id / aph(3'')-Ib / sul2 / catA1 / tet(B) / blaTEM-1B / oqxB / oqxA / sitABCD	iroN / iroE / iroD / iroC / iroB / iucA / iucB / iucC / iucD / iutA	1..125272	125272

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T72096 WP_001312861.1 NZ_CP019268:114221-114379 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T72096 NZ_CP019268:114221-114379 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 64 bp

>AT72096 NZ_CP019268:c114172-114109 [Escherichia coli]
GACTAGACATAGGGATGCCTCGTGGTGGTTAATGAAAATTAACTTACTACGGGGCTATCTTCTT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin