Detailed information of TA system TA072077

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ralRA/-
• Location: 2751291..2751662
• Replicon: chromosome
• Accession: NZ_CP019267
• Organism: Escherichia coli strain 13C1079T

Toxin (Protein)

• Gene name: ralR
• Uniprot ID: F4VC37
• Locus tag: BWI87_RS14125
• Protein ID: WP_001317028.1
• Coordinates: 2751468..2751662 (-)
• Length: 65 a.a.

Antitoxin (RNA)

Gene name	ralA
Locus tag	-
Coordinates	2751291..2751469 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BWI87_RS14095	2747043..2747216	+	174	WP_001296046.1	hypothetical protein	-
BWI87_RS14100	2747246..2748619	+	1374	WP_000123745.1	ATP-dependent RNA helicase DbpA	-
BWI87_RS14105	2748748..2749683	-	936	WP_001153728.1	tRNA 2-thiocytidine(32) synthetase TtcA	-
BWI87_RS14110	2749735..2750970	-	1236	WP_000040852.1	site-specific integrase	-
BWI87_RS14115	2750972..2751187	-	216	WP_000079604.1	excisionase XisR	-
BWI87_RS14120	2751266..2751475	-	210	WP_000276809.1	double-strand break reduction protein RcbA	-
-	2751291..2751469	+	179	NuclAT_0	-	Antitoxin
-	2751291..2751469	+	179	NuclAT_0	-	Antitoxin
-	2751291..2751469	+	179	NuclAT_0	-	Antitoxin
-	2751291..2751469	+	179	NuclAT_0	-	Antitoxin
BWI87_RS14125	2751468..2751662	-	195	WP_001317028.1	type I toxin-antitoxin system endodeoxyribonuclease toxin RalR	Toxin
BWI87_RS14130	2751719..2752528	-	810	WP_000166319.1	recombination protein RecT	-
BWI87_RS14135	2752521..2755121	-	2601	WP_001532611.1	exodeoxyribonuclease VIII	-
BWI87_RS14140	2755223..2755498	-	276	WP_000632297.1	protein RacC	-
BWI87_RS14145	2755573..2755743	-	171	WP_001352098.1	conserved protein, Rac prophage	-
BWI87_RS14150	2755743..2755964	-	222	WP_000560225.1	killing protein KilR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2749735..2780302	30567

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endodeoxyribonuclease toxin RalR (6-54)

Sequences

Toxin

Protein

• Length: 65 a.a.
• Molecular weight: 7018.89 Da
• Isoelectric Point: 8.9538

>T72077 WP_001317028.1 NZ_CP019267:c2751662-2751468 [Escherichia coli]
MRYDNVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALERWNKRTTGNNNGGVHV

Nucleotide

• Length: 195 bp

>T72077 NZ_CP019267:c2751662-2751468 [Escherichia coli]
ATGAGATATGACAATGTTAAACCATGTCCATTTTGTGGTTGTCCATCAGTAACGGTGAAAGCCATTTCAGGATATTACCG
AGCGAAGTGTAACGGATGCGAATCCCGAACCGGTTATGGTGGAAGTGAAAAAGAAGCACTCGAAAGATGGAATAAACGAA
CCACTGGAAATAATAATGGAGGTGTTCATGTATAA

Antitoxin

Nucleotide

• Length: 179 bp

>AT72077 NZ_CP019267:2751291-2751469 [Escherichia coli]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Structures

Toxin

• AlphaFold DB: A0A7L6LHV7

Antitoxin