Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type I Classification (family/domain) hok-sok/-
Location 65892..66318 Replicon plasmid p13C1065T-2
Accession NZ_CP019261
Organism Escherichia coli strain 13C1065T

Toxin (Protein)


Gene name hok Uniprot ID P11895
Locus tag BWI86_RS25980 Protein ID WP_001312861.1
Coordinates 65892..66050 (-) Length 53 a.a.

Antitoxin (RNA)


Gene name sok
Locus tag -
Coordinates 66094..66318 (-)

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BWI86_RS25940 61263..61952 - 690 WP_039002187.1 conjugal transfer transcriptional regulator TraJ -
BWI86_RS25945 62139..62522 - 384 WP_001151524.1 relaxosome protein TraM -
BWI86_RS25950 62843..63445 + 603 WP_000243712.1 transglycosylase SLT domain-containing protein -
BWI86_RS25955 63742..64563 - 822 WP_072795862.1 DUF945 domain-containing protein -
BWI86_RS25960 64683..64970 - 288 WP_000107535.1 hypothetical protein -
BWI86_RS25980 65892..66050 - 159 WP_001312861.1 type I toxin-antitoxin system Hok family toxin Toxin
- 66094..66318 - 225 NuclAT_0 - Antitoxin
- 66094..66318 - 225 NuclAT_0 - Antitoxin
- 66094..66318 - 225 NuclAT_0 - Antitoxin
- 66094..66318 - 225 NuclAT_0 - Antitoxin
BWI86_RS28840 66130..66318 + 189 WP_001299721.1 hypothetical protein -
BWI86_RS25985 66330..67049 - 720 WP_001276232.1 plasmid SOS inhibition protein A -
BWI86_RS25990 67046..67480 - 435 WP_000845953.1 conjugation system SOS inhibitor PsiB -
BWI86_RS25995 67535..69493 - 1959 WP_039000998.1 ParB/RepB/Spo0J family partition protein -
BWI86_RS26000 69558..69791 - 234 WP_000005987.1 DUF905 family protein -
BWI86_RS26005 69848..70387 - 540 WP_032332913.1 single-stranded DNA-binding protein -
BWI86_RS29230 70413..70619 - 207 WP_000275853.1 hypothetical protein -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid floR / sul2 / aph(3')-Ia / sul3 / blaTEM-1B / lnu(F) / ant(3'')-Ia / aac(3)-IId / tet(M) / cmlA1 / aadA2 / dfrA12 - 1..138610 138610


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(8-48)


Sequences


Toxin        


Download         Length: 53 a.a.        Molecular weight: 6082.32 Da        Isoelectric Point: 9.2584

>T72056 WP_001312861.1 NZ_CP019261:c66050-65892 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Download         Length: 159 bp

>T72056 NZ_CP019261:c66050-65892 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin


Download         Length: 225 bp

>AT72056 NZ_CP019261:c66318-66094 [Escherichia coli]
TCACACGGATTTCCCGTGAACGGTCTGAATGAGCGGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB P11895


Antitoxin

Download structure file

References