Detailed information of TA system TA072052

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 30595..30849
• Replicon: plasmid p13C1065T-2
• Accession: NZ_CP019261
• Organism: Escherichia coli strain 13C1065T

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: -
• Locus tag: BWI86_RS25780
• Protein ID: WP_001351576.1
• Coordinates: 30595..30801 (-)
• Length: 69 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	30788..30849 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BWI86_RS25730	26617..27117	+	501	Protein_31	Tn3 family transposase	-
BWI86_RS25735	27151..27339	+	189	Protein_32	IS3 family transposase	-
BWI86_RS25740	27414..27701	-	288	WP_000222766.1	type II toxin-antitoxin system RelE/ParE family toxin	-
BWI86_RS25745	27698..27949	-	252	WP_001132900.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
BWI86_RS25760	28917..29771	-	855	Protein_35	incFII family plasmid replication initiator RepA	-
BWI86_RS25765	29764..29838	-	75	WP_061357229.1	RepA leader peptide Tap	-
BWI86_RS25775	30070..30327	-	258	WP_001541895.1	replication regulatory protein RepA	-
BWI86_RS25780	30595..30801	-	207	WP_001351576.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	30788..30849	+	62	NuclAT_1	-	Antitoxin
-	30788..30849	+	62	NuclAT_1	-	Antitoxin
-	30788..30849	+	62	NuclAT_1	-	Antitoxin
-	30788..30849	+	62	NuclAT_1	-	Antitoxin
BWI86_RS25790	31191..31403	-	213	WP_001541896.1	ANR family transcriptional regulator	-
BWI86_RS25795	31538..32098	-	561	WP_000139310.1	fertility inhibition protein FinO	-
BWI86_RS25800	32153..32896	-	744	WP_039002216.1	conjugal transfer pilus acetylase TraX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	floR / sul2 / aph(3')-Ia / sul3 / blaTEM-1B / lnu(F) / ant(3'')-Ia / aac(3)-IId / tet(M) / cmlA1 / aadA2 / dfrA12	-	1..138610	138610

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (23-64)

Sequences

Toxin

Protein

• Length: 69 a.a.
• Molecular weight: 7826.31 Da
• Isoelectric Point: 8.8807

>T72052 WP_001351576.1 NZ_CP019261:c30801-30595 [Escherichia coli]
MKYLNTTDCSLFFAERSKFMTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 207 bp

>T72052 NZ_CP019261:c30801-30595 [Escherichia coli]
ATGAAGTACCTTAACACTACTGATTGTAGCCTCTTCTTTGCAGAGAGGTCAAAGTTTATGACGAAATATGCCCTTATCGG
GTTGCTCGCCGTGTGCGCCACTGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTTATGTGAGCTGAATATTCACAGAG
GAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Nucleotide

• Length: 62 bp

>AT72052 NZ_CP019261:30788-30849 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Antitoxin