72049

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4610614..4611123 Replicon chromosome
Accession NZ_CP019259
Organism Escherichia coli strain 13C1065T

Toxin (Protein)


Gene name ghoT Uniprot ID -
Locus tag BWI86_RS23865 Protein ID WP_089514152.1
Coordinates 4610614..4610799 (-) Length 62 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag BWI86_RS23870 Protein ID WP_000398619.1
Coordinates 4610827..4611123 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BWI86_RS23855 4607297..4608754 + 1458 WP_000856841.1 dipeptide/tripeptide permease DtpC -
BWI86_RS23860 4608991..4610508 + 1518 WP_001295074.1 lysine--tRNA ligase -
BWI86_RS23865 4610614..4610799 - 186 WP_089514152.1 type V toxin-antitoxin system toxin GhoT Toxin
BWI86_RS23870 4610827..4611123 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
BWI86_RS23880 4611350..4611622 - 273 WP_000405647.1 N-acetyltransferase -
BWI86_RS23885 4611634..4611864 - 231 WP_000371704.1 (4Fe-4S)-binding protein -
BWI86_RS23890 4612045..4613676 + 1632 WP_001565376.1 sensor histidine kinase -
BWI86_RS23895 4613673..4614392 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 4600945..4611123 10178


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-54)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 62 a.a.        Molecular weight: 6988.54 Da        Isoelectric Point: 10.2380

>T72049 WP_089514152.1 NZ_CP019259:c4610799-4610614 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFLSFRSCS

Download         Length: 186 bp

>T72049 NZ_CP019259:c4610799-4610614 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTCTCTCTTTTAGGAGCTGTAGTTGA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT72049 WP_000398619.1 NZ_CP019259:c4611123-4610827 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT72049 NZ_CP019259:c4611123-4610827 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTAATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBH6

References