Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 4084569..4085110 Replicon chromosome
Accession NZ_CP019259
Organism Escherichia coli strain 13C1065T

Toxin (Protein)


Gene name yafQ Uniprot ID -
Locus tag BWI86_RS21180 Protein ID WP_072645854.1
Coordinates 4084832..4085110 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag BWI86_RS21175 Protein ID WP_000729704.1
Coordinates 4084569..4084829 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BWI86_RS21155 4080244..4081029 - 786 WP_000207587.1 putative lateral flagellar export/assembly protein LafU -
BWI86_RS21160 4081001..4082713 + 1713 Protein_3827 flagellar biosynthesis protein FlhA -
BWI86_RS21165 4082937..4083434 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
BWI86_RS21170 4083610..4084383 - 774 WP_015675129.1 C40 family peptidase -
BWI86_RS21175 4084569..4084829 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
BWI86_RS21180 4084832..4085110 + 279 WP_072645854.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
BWI86_RS21185 4085266..4086006 + 741 WP_001225679.1 murein L,D-transpeptidase -
BWI86_RS21190 4085977..4086744 - 768 WP_000333379.1 class II glutamine amidotransferase -
BWI86_RS21195 4086950..4087528 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(5-92)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10824.58 Da        Isoelectric Point: 10.0703

>T72047 WP_072645854.1 NZ_CP019259:4084832-4085110 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYHMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T72047 NZ_CP019259:4084832-4085110 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCATATGACGCTTCTTATCAATAATGCTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT72047 WP_000729704.1 NZ_CP019259:4084569-4084829 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT72047 NZ_CP019259:4084569-4084829 [Escherichia coli]
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References