Detailed information of TA system TA072046

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 4078372..4079066
• Replicon: chromosome
• Accession: NZ_CP019259
• Organism: Escherichia coli strain 13C1065T

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1EXB8
• Locus tag: BWI86_RS21140
• Protein ID: WP_001263493.1
• Coordinates: 4078372..4078770 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: BWI86_RS21145
• Protein ID: WP_000554757.1
• Coordinates: 4078773..4079066 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BWI86_RS21110	4073372..4074616	-	1245	WP_000189541.1	esterase FrsA	-
-	4074032..4074112	-	81	NuclAT_10	-	-
-	4074032..4074112	-	81	NuclAT_10	-	-
-	4074032..4074112	-	81	NuclAT_10	-	-
-	4074032..4074112	-	81	NuclAT_10	-	-
BWI86_RS21115	4074708..4075166	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
BWI86_RS21120	4075427..4076884	+	1458	WP_001293003.1	cytosol nonspecific dipeptidase	-
BWI86_RS21125	4076941..4077462	-	522	Protein_3820	peptide chain release factor H	-
BWI86_RS21130	4077461..4077664	-	204	Protein_3821	RNA ligase RtcB family protein	-
BWI86_RS21135	4077910..4078362	-	453	WP_001059847.1	GNAT family N-acetyltransferase	-
BWI86_RS21140	4078372..4078770	-	399	WP_001263493.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
BWI86_RS21145	4078773..4079066	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
BWI86_RS21150	4079118..4080173	-	1056	WP_001226164.1	DNA polymerase IV	-
BWI86_RS21155	4080244..4081029	-	786	WP_000207587.1	putative lateral flagellar export/assembly protein LafU	-
BWI86_RS21160	4081001..4082713	+	1713	Protein_3827	flagellar biosynthesis protein FlhA	-
BWI86_RS21165	4082937..4083434	-	498	WP_000006255.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	4027043..4084236	57193

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15472.83 Da
• Isoelectric Point: 8.0949

>T72046 WP_001263493.1 NZ_CP019259:c4078770-4078372 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T72046 NZ_CP019259:c4078770-4078372 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACAGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT72046 WP_000554757.1 NZ_CP019259:c4079066-4078773 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT72046 NZ_CP019259:c4079066-4078773 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATTACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: S1EXB8

Antitoxin

• AlphaFold DB: S1FJN6