Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2192781..2193283 Replicon chromosome
Accession NZ_CP019259
Organism Escherichia coli strain 13C1065T

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag BWI86_RS11025 Protein ID WP_000767829.1
Coordinates 2193029..2193283 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag BWI86_RS11020 Protein ID WP_001259255.1
Coordinates 2192781..2193032 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BWI86_RS10990 2187959..2189026 - 1068 WP_000080085.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
BWI86_RS10995 2189026..2190096 - 1071 WP_000108940.1 histidinol-phosphate transaminase -
BWI86_RS11000 2190093..2191397 - 1305 WP_001515511.1 histidinol dehydrogenase -
BWI86_RS11005 2191403..2192302 - 900 WP_000131776.1 ATP phosphoribosyltransferase -
BWI86_RS11015 2192448..2192498 - 51 WP_001364200.1 his operon leader peptide -
BWI86_RS11020 2192781..2193032 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
BWI86_RS11025 2193029..2193283 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
BWI86_RS11030 2193366..2194190 + 825 WP_000754749.1 SDR family oxidoreductase -
BWI86_RS11035 2194236..2195165 + 930 WP_000803351.1 LysR family transcriptional regulator -
BWI86_RS11040 2195380..2195442 + 63 WP_010723108.1 membrane protein YoeI -
BWI86_RS11045 2195432..2196790 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
BWI86_RS11050 2196969..2198027 + 1059 WP_000492315.1 transport protein YeeE -
BWI86_RS11055 2198041..2198268 + 228 WP_000985280.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T72033 WP_000767829.1 NZ_CP019259:2193029-2193283 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T72033 NZ_CP019259:2193029-2193283 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT72033 WP_001259255.1 NZ_CP019259:2192781-2193032 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT72033 NZ_CP019259:2192781-2193032 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References