Detailed information of TA system TA072028

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 1079594..1080393
• Replicon: chromosome
• Accession: NZ_CP019259
• Organism: Escherichia coli strain 13C1065T

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: -
• Locus tag: BWI86_RS05460
• Protein ID: WP_032221941.1
• Coordinates: 1079594..1080058 (-)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: S1EB98
• Locus tag: BWI86_RS05465
• Protein ID: WP_001307405.1
• Coordinates: 1080058..1080393 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BWI86_RS05425	1074595..1075029	-	435	WP_000948824.1	PTS sugar transporter subunit IIA	-
BWI86_RS05430	1075047..1075925	-	879	WP_001300474.1	PTS system mannose/fructose/sorbose family transporter subunit IID	-
BWI86_RS05435	1075915..1076694	-	780	WP_000406214.1	PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC	-
BWI86_RS05440	1076705..1077178	-	474	WP_001295547.1	PTS N-acetylgalactosamine transporter subunit IIB	-
BWI86_RS05445	1077201..1078481	-	1281	WP_000681903.1	tagatose-bisphosphate aldolase subunit KbaZ	-
BWI86_RS05455	1078730..1079539	+	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
BWI86_RS05460	1079594..1080058	-	465	WP_032221941.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
BWI86_RS05465	1080058..1080393	-	336	WP_001307405.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
BWI86_RS05470	1080542..1082113	-	1572	WP_001723936.1	galactarate dehydratase	-
BWI86_RS05475	1082488..1083822	+	1335	WP_148116239.1	galactarate/glucarate/glycerate transporter GarP	-
BWI86_RS05480	1083838..1084608	+	771	WP_001723934.1	2-dehydro-3-deoxyglucarate aldolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17808.24 Da
• Isoelectric Point: 9.6804

>T72028 WP_032221941.1 NZ_CP019259:c1080058-1079594 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQKKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Nucleotide

• Length: 465 bp

>T72028 NZ_CP019259:c1080058-1079594 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAAAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAGACAGAAGAAACCCATTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12358.94 Da
• Isoelectric Point: 4.8616

>AT72028 WP_001307405.1 NZ_CP019259:c1080393-1080058 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT72028 NZ_CP019259:c1080393-1080058 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Structures

Antitoxin

• AlphaFold DB: A0A0E0XVC7