Detailed information of TA system TA072011

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 1467980..1468582
• Replicon: chromosome
• Accession: NZ_CP019256
• Organism: Escherichia coli strain 13TMH22

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: BWI85_RS07720
• Protein ID: WP_000897305.1
• Coordinates: 1468271..1468582 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: BWI85_RS07715
• Protein ID: WP_000356397.1
• Coordinates: 1467980..1468270 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BWI85_RS07685	1463906..1464808	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
BWI85_RS07690	1464805..1465440	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
BWI85_RS07695	1465437..1466366	+	930	WP_148124224.1	formate dehydrogenase accessory protein FdhE	-
BWI85_RS07700	1466696..1466938	-	243	WP_001086388.1	hypothetical protein	-
BWI85_RS07705	1467157..1467375	-	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
BWI85_RS07715	1467980..1468270	-	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
BWI85_RS07720	1468271..1468582	-	312	WP_000897305.1	hypothetical protein	Toxin
BWI85_RS07725	1468811..1469719	+	909	WP_001162704.1	alpha/beta hydrolase	-
BWI85_RS07730	1469783..1470724	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
BWI85_RS07735	1470769..1471206	-	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
BWI85_RS07740	1471203..1472075	-	873	WP_000920762.1	virulence factor BrkB family protein	-
BWI85_RS07745	1472069..1472668	-	600	WP_001315111.1	glucose-1-phosphatase	-
BWI85_RS07750	1472767..1473552	-	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T72011 WP_000897305.1 NZ_CP019256:c1468582-1468271 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T72011 NZ_CP019256:c1468582-1468271 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT72011 WP_000356397.1 NZ_CP019256:c1468270-1467980 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT72011 NZ_CP019256:c1468270-1467980 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7