Detailed information of TA system TA071992

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 122874..123144
• Replicon: plasmid p13KWH46-1
• Accession: NZ_CP019251
• Organism: Escherichia coli strain 13KWH46

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: BWI84_RS26055
• Protein ID: WP_001312861.1
• Coordinates: 122986..123144 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	122874..122937 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BWI84_RS26015	118094..119065	+	972	WP_000817028.1	ParB/RepB/Spo0J family plasmid partition protein	-
BWI84_RS29155	120204..120410	+	207	WP_000275856.1	hypothetical protein	-
BWI84_RS26030	120436..120975	+	540	WP_000290840.1	single-stranded DNA-binding protein	-
BWI84_RS26035	121043..121276	+	234	WP_000005987.1	DUF905 family protein	-
BWI84_RS26040	121304..121501	+	198	Protein_123	hypothetical protein	-
BWI84_RS26045	121556..121990	+	435	WP_000845936.1	conjugation system SOS inhibitor PsiB	-
BWI84_RS26050	121987..122706	+	720	WP_001276238.1	plasmid SOS inhibition protein A	-
BWI84_RS28930	122718..122906	-	189	WP_001299721.1	hypothetical protein	-
-	122718..122942	+	225	NuclAT_0	-	-
-	122718..122942	+	225	NuclAT_0	-	-
-	122718..122942	+	225	NuclAT_0	-	-
-	122718..122942	+	225	NuclAT_0	-	-
-	122874..122937	-	64	-	-	Antitoxin
BWI84_RS26055	122986..123144	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
BWI84_RS26070	124065..124352	+	288	WP_000107535.1	hypothetical protein	-
BWI84_RS26075	124470..125291	+	822	WP_001234426.1	DUF945 domain-containing protein	-
BWI84_RS26080	125588..126190	-	603	WP_000243712.1	transglycosylase SLT domain-containing protein	-
BWI84_RS26085	126511..126894	+	384	WP_001151524.1	relaxosome protein TraM	-
BWI84_RS26090	127081..127770	+	690	WP_000283385.1	conjugal transfer transcriptional regulator TraJ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aph(3')-Ia / aph(6)-Id / aph(3'')-Ib / sul2 / blaTEM-1B / tet(B) / catA1 / oqxB / oqxA / sitABCD	iroN / iroE / iroD / iroC / iroB / iucA / iucB / iucC / iucD / iutA	1..162357	162357

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T71992 WP_001312861.1 NZ_CP019251:122986-123144 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T71992 NZ_CP019251:122986-123144 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 64 bp

>AT71992 NZ_CP019251:c122937-122874 [Escherichia coli]
GACTAGACATAGGGATGCCTCGTGGTGGTTAATGAAAATTAACTTACTACGGGGCTATCTTCTT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin