Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 4615754..4616295 Replicon chromosome
Accession NZ_CP019250
Organism Escherichia coli strain 13KWH46

Toxin (Protein)


Gene name yafQ Uniprot ID E9TC49
Locus tag BWI84_RS23985 Protein ID WP_000615982.1
Coordinates 4616017..4616295 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag BWI84_RS23980 Protein ID WP_000729704.1
Coordinates 4615754..4616014 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BWI84_RS23955 4611537..4612322 - 786 WP_000207575.1 putative lateral flagellar export/assembly protein LafU -
BWI84_RS23960 4612294..4614006 + 1713 Protein_4367 flagellar biosynthesis protein FlhA -
BWI84_RS23965 4614111..4614389 + 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
BWI84_RS23970 4614382..4614738 + 357 WP_001030484.1 type II toxin-antitoxin system HicB family antitoxin -
BWI84_RS23975 4614795..4615568 - 774 WP_000543899.1 C40 family peptidase -
BWI84_RS23980 4615754..4616014 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
BWI84_RS23985 4616017..4616295 + 279 WP_000615982.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
BWI84_RS23990 4616451..4617191 + 741 WP_001225679.1 murein L,D-transpeptidase -
BWI84_RS23995 4617162..4617929 - 768 WP_000333380.1 class II glutamine amidotransferase -
BWI84_RS24000 4618135..4618713 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10816.59 Da        Isoelectric Point: 10.0702

>T71990 WP_000615982.1 NZ_CP019250:4616017-4616295 [Escherichia coli]
MIQRDIEYSGQFSKDVKVAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T71990 NZ_CP019250:4616017-4616295 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAAGTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT71990 WP_000729704.1 NZ_CP019250:4615754-4616014 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT71990 NZ_CP019250:4615754-4616014 [Escherichia coli]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References