Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2648605..2649107 Replicon chromosome
Accession NZ_CP019250
Organism Escherichia coli strain 13KWH46

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag BWI84_RS13645 Protein ID WP_000767829.1
Coordinates 2648853..2649107 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag BWI84_RS13640 Protein ID WP_001259255.1
Coordinates 2648605..2648856 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BWI84_RS13610 2643783..2644850 - 1068 WP_000080093.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
BWI84_RS13615 2644850..2645920 - 1071 WP_000108950.1 histidinol-phosphate transaminase -
BWI84_RS13620 2645917..2647221 - 1305 WP_001499066.1 histidinol dehydrogenase -
BWI84_RS13625 2647227..2648126 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
BWI84_RS13635 2648272..2648322 - 51 WP_001364200.1 his operon leader peptide -
BWI84_RS13640 2648605..2648856 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
BWI84_RS13645 2648853..2649107 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
BWI84_RS13650 2649190..2650014 + 825 WP_000754749.1 SDR family oxidoreductase -
BWI84_RS13655 2650060..2650989 + 930 WP_000803351.1 LysR family transcriptional regulator -
BWI84_RS13660 2651204..2651266 + 63 WP_010723108.1 membrane protein YoeI -
BWI84_RS13665 2651256..2652614 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
BWI84_RS13670 2652793..2653851 + 1059 WP_023568741.1 transport protein YeeE -
BWI84_RS13675 2653865..2654092 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T71978 WP_000767829.1 NZ_CP019250:2648853-2649107 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T71978 NZ_CP019250:2648853-2649107 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT71978 WP_001259255.1 NZ_CP019250:2648605-2648856 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT71978 NZ_CP019250:2648605-2648856 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References