Detailed information of TA system TA071961

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 112821..113091
• Replicon: plasmid pCombat13F7-2
• Accession: NZ_CP019247
• Organism: Escherichia coli strain Combat13F7

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: BWI83_RS25385
• Protein ID: WP_001312861.1
• Coordinates: 112933..113091 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	112821..112884 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BWI83_RS25340	107870..108142	+	273	WP_072133095.1	hypothetical protein	-
BWI83_RS25360	108615..109142	+	528	WP_000290793.1	single-stranded DNA-binding protein	-
BWI83_RS25365	109198..109431	+	234	WP_000006004.1	DUF905 domain-containing protein	-
BWI83_RS25370	109490..111448	+	1959	WP_001145469.1	ParB/RepB/Spo0J family partition protein	-
BWI83_RS25375	111503..111937	+	435	WP_000845895.1	conjugation system SOS inhibitor PsiB	-
BWI83_RS25380	111934..112653	+	720	WP_001276228.1	plasmid SOS inhibition protein A	-
BWI83_RS26830	112665..112853	-	189	WP_001299721.1	hypothetical protein	-
-	112665..112889	+	225	NuclAT_0	-	-
-	112665..112889	+	225	NuclAT_0	-	-
-	112665..112889	+	225	NuclAT_0	-	-
-	112665..112889	+	225	NuclAT_0	-	-
-	112821..112884	-	64	-	-	Antitoxin
BWI83_RS25385	112933..113091	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
BWI83_RS26980	113782..113988	+	207	WP_000547939.1	hypothetical protein	-
BWI83_RS25405	114013..114300	+	288	WP_000107535.1	hypothetical protein	-
BWI83_RS25410	114423..115244	+	822	WP_001234469.1	DUF945 domain-containing protein	-
BWI83_RS25415	115541..116188	-	648	WP_000614936.1	transglycosylase SLT domain-containing protein	-
BWI83_RS25420	116465..116848	+	384	WP_000124981.1	relaxosome protein TraM	-
BWI83_RS25425	117039..117725	+	687	WP_000332484.1	PAS domain-containing protein	-
BWI83_RS25430	117819..118046	+	228	WP_001254386.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	dfrA1 / aadA1 / qacE / sul1 / mph(A) / aph(3'')-Ib / aph(6)-Id / blaTEM-1B / oqxB / oqxA / tet(A) / sitABCD	iroN / iroE / iroD / iroC / iroB	1..151049	151049

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T71961 WP_001312861.1 NZ_CP019247:112933-113091 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T71961 NZ_CP019247:112933-113091 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 64 bp

>AT71961 NZ_CP019247:c112884-112821 [Escherichia coli]
GACTAGACATAGGGATGCCTCGTGGTGGTTAATGAAAATTAACTTACTACGGGGCTATCTTCTT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin