Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 3493812..3494314 Replicon chromosome
Accession NZ_CP019245
Organism Escherichia coli strain Combat13F7

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag BWI83_RS17735 Protein ID WP_000767829.1
Coordinates 3494060..3494314 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag BWI83_RS17730 Protein ID WP_001259255.1
Coordinates 3493812..3494063 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BWI83_RS17700 3488991..3490058 - 1068 WP_000080102.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
BWI83_RS17705 3490058..3491128 - 1071 WP_061354393.1 histidinol-phosphate transaminase -
BWI83_RS17710 3491125..3492429 - 1305 WP_032172814.1 histidinol dehydrogenase -
BWI83_RS17715 3492435..3493334 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
BWI83_RS17725 3493480..3493530 - 51 WP_001364200.1 his operon leader peptide -
BWI83_RS17730 3493812..3494063 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
BWI83_RS17735 3494060..3494314 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
BWI83_RS17740 3494397..3495221 + 825 WP_029399144.1 SDR family oxidoreductase -
BWI83_RS17745 3495267..3496196 + 930 WP_000803351.1 LysR family transcriptional regulator -
BWI83_RS17750 3496411..3496473 + 63 WP_010723108.1 membrane protein YoeI -
BWI83_RS17755 3496463..3497821 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
BWI83_RS17760 3498000..3499058 + 1059 WP_032352489.1 transport protein YeeE -
BWI83_RS17765 3499072..3499299 + 228 WP_097430815.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T71950 WP_000767829.1 NZ_CP019245:3494060-3494314 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T71950 NZ_CP019245:3494060-3494314 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT71950 WP_001259255.1 NZ_CP019245:3493812-3494063 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT71950 NZ_CP019245:3493812-3494063 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References