Detailed information of TA system TA071937

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 27570..27840
• Replicon: plasmid pCombat2C1-1
• Accession: NZ_CP019244
• Organism: Escherichia coli strain Combat2C1

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: BWI82_RS27065
• Protein ID: WP_001312861.1
• Coordinates: 27682..27840 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	27570..27633 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BWI82_RS27020	22624..22878	+	255	WP_072254791.1	hypothetical protein	-
BWI82_RS27825	23119..23325	+	207	WP_000547944.1	hypothetical protein	-
BWI82_RS27040	23351..23884	+	534	WP_105906671.1	single-stranded DNA-binding protein	-
BWI82_RS27045	23942..24175	+	234	WP_000005998.1	DUF905 family protein	-
BWI82_RS27050	24239..26197	+	1959	WP_105272432.1	ParB/RepB/Spo0J family partition protein	-
BWI82_RS27055	26252..26686	+	435	WP_001470779.1	conjugation system SOS inhibitor PsiB	-
BWI82_RS27060	26683..27402	+	720	WP_105906669.1	plasmid SOS inhibition protein A	-
BWI82_RS27610	27414..27602	-	189	WP_001299721.1	hypothetical protein	-
-	27414..27638	+	225	NuclAT_0	-	-
-	27414..27638	+	225	NuclAT_0	-	-
-	27414..27638	+	225	NuclAT_0	-	-
-	27414..27638	+	225	NuclAT_0	-	-
-	27570..27633	-	64	-	-	Antitoxin
BWI82_RS27065	27682..27840	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
BWI82_RS27830	28141..28437	-	297	Protein_41	hypothetical protein	-
BWI82_RS27835	28507..28710	+	204	Protein_42	single-stranded DNA-binding protein	-
BWI82_RS27080	28733..29056	+	324	WP_000533253.1	hypothetical protein	-
BWI82_RS27085	29115..29357	+	243	WP_000540591.1	hypothetical protein	-
BWI82_RS27105	30280..30576	+	297	WP_001272251.1	hypothetical protein	-
BWI82_RS27110	30687..31508	+	822	WP_021553189.1	DUF945 domain-containing protein	-
BWI82_RS27115	31804..32313	-	510	WP_089576520.1	transglycosylase SLT domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	oqxB / oqxA	-	1..71430	71430

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T71937 WP_001312861.1 NZ_CP019244:27682-27840 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T71937 NZ_CP019244:27682-27840 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 64 bp

>AT71937 NZ_CP019244:c27633-27570 [Escherichia coli]
GACTAGACATAGGGATGCCTCGTGGTGGTTAATGAAAATTAACTTACTACGGGGCTATCTTCTT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin