Detailed information of TA system TA071922

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 3198766..3199368
• Replicon: chromosome
• Accession: NZ_CP019243
• Organism: Escherichia coli strain Combat2C1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: BWI82_RS16420
• Protein ID: WP_000897305.1
• Coordinates: 3198766..3199077 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: BWI82_RS16425
• Protein ID: WP_000356397.1
• Coordinates: 3199078..3199368 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BWI82_RS16395	3194680..3195279	+	600	WP_001298594.1	glucose-1-phosphatase	-
BWI82_RS16400	3195273..3196145	+	873	WP_000920762.1	virulence factor BrkB family protein	-
BWI82_RS16405	3196142..3196579	+	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
BWI82_RS16410	3196624..3197565	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
BWI82_RS16415	3197629..3198537	-	909	WP_001298596.1	alpha/beta hydrolase	-
BWI82_RS16420	3198766..3199077	+	312	WP_000897305.1	hypothetical protein	Toxin
BWI82_RS16425	3199078..3199368	+	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
BWI82_RS16435	3199973..3200191	+	219	WP_001298592.1	ribbon-helix-helix domain-containing protein	-
BWI82_RS16440	3200406..3201335	-	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
BWI82_RS16445	3201332..3201967	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
BWI82_RS16450	3201964..3202866	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12172.16 Da
• Isoelectric Point: 9.7791

>T71922 WP_000897305.1 NZ_CP019243:3198766-3199077 [Escherichia coli]
ILFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T71922 NZ_CP019243:3198766-3199077 [Escherichia coli]
ATCCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT71922 WP_000356397.1 NZ_CP019243:3199078-3199368 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT71922 NZ_CP019243:3199078-3199368 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7