Detailed information of TA system TA071847

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 3795484..3796081
• Replicon: chromosome
• Accession: NZ_CP019219
• Organism: Klebsiella pneumoniae strain 1756

Toxin (Protein)

• Gene name: higB
• Uniprot ID: R4YIC5
• Locus tag: BWG70_RS19515
• Protein ID: WP_004142563.1
• Coordinates: 3795764..3796081 (-)
• Length: 106 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: BWG70_RS19510
• Protein ID: WP_076027550.1
• Coordinates: 3795484..3795771 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BWG70_RS19480	3791565..3791813	+	249	WP_002893037.1	DUF1158 domain-containing protein	-
BWG70_RS19485	3791831..3792172	-	342	WP_002893035.1	RamA family antibiotic efflux transcriptional regulator	-
BWG70_RS19490	3792203..3793318	-	1116	WP_076027549.1	MBL fold metallo-hydrolase	-
BWG70_RS19495	3793498..3794079	+	582	WP_004176968.1	TetR/AcrR family transcriptional regulator	-
BWG70_RS19500	3794079..3794447	+	369	WP_004142557.1	MmcQ/YjbR family DNA-binding protein	-
BWG70_RS19505	3794567..3795220	+	654	WP_004178896.1	oxygen-insensitive NAD(P)H nitroreductase	-
BWG70_RS19510	3795484..3795771	-	288	WP_076027550.1	helix-turn-helix transcriptional regulator	Antitoxin
BWG70_RS19515	3795764..3796081	-	318	WP_004142563.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BWG70_RS19520	3796266..3797309	-	1044	WP_048336957.1	DUF2157 domain-containing protein	-
BWG70_RS19530	3797975..3798841	-	867	WP_004151823.1	helix-turn-helix transcriptional regulator	-
BWG70_RS19535	3798950..3800377	+	1428	WP_004176980.1	MFS transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-100)

Antitoxin

• Cro/C1-type helix-turn-helix domain (38-88)

Sequences

Toxin

Protein

• Length: 106 a.a.
• Molecular weight: 12110.39 Da
• Isoelectric Point: 11.2767

>T71847 WP_004142563.1 NZ_CP019219:c3796081-3795764 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPPIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Nucleotide

• Length: 318 bp

>T71847 NZ_CP019219:c3796081-3795764 [Klebsiella pneumoniae]
ATGTTCAGATTGGTTGTGCATGTGGACGTTAAAAAGGAATTGCAGGCGCTTCCGCCCATTGTCCAGGCGAAGATGATCCG
GCAATTAGACAAGTTACGCCAAAATCCCACCGCGCTGAGAGAACCTGACAGTAAACCGCTGGGGCAGGGGCTATTTGAAA
TTCGTGCGTTAGGTTCGGTACAGGGGCGAGGGATCTACGTTTTTCAGCAAGGCAAGACGCTCTTTCTGCTACGGGTTTTT
GTCAAGAAAACGCAAAAAACCCCGTCATCGGAAATTCGCCTGGCGCTGAAGCGATTAGAGGAGATGCGCAATGAGTAA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10783.51 Da
• Isoelectric Point: 10.2212

>AT71847 WP_076027550.1 NZ_CP019219:c3795771-3795484 [Klebsiella pneumoniae]
MSKKIIDWDTLKIELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Nucleotide

• Length: 288 bp

>AT71847 NZ_CP019219:c3795771-3795484 [Klebsiella pneumoniae]
ATGAGTAAAAAGATCATTGACTGGGATACGTTAAAAATAGAACTGCTTGGCCAGCCTGAGGTTCAGGCTGCATTCGATGC
GCAAGAACGTCAACAGCGATTACGTACTATGCTGGCGCAATGGCGCGAGCAGGCGGGGCTAACCCGAGCCCAGGTGGCGC
AGAGGATGGGGGTGAGTGCGCCGACGGTCTCCAGAATGGAAGCCAATATCACCCGCGCCAGTCTGGATACCTTAACGCGA
TACGCGTTAGCCTGCGGTATAAAGCACCCGCAAGTTTTACTTTACTAA

Structures

Toxin

• AlphaFold DB: A0A0M5MXH8