Detailed information of TA system TA071835

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 358323..358969
• Replicon: chromosome
• Accession: NZ_CP019219
• Organism: Klebsiella pneumoniae strain 1756

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A483JFR4
• Locus tag: BWG70_RS01735
• Protein ID: WP_004188313.1
• Coordinates: 358323..358670 (+)
• Length: 116 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: BWG70_RS01740
• Protein ID: WP_004210373.1
• Coordinates: 358670..358969 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BWG70_RS01725	354259..355692	+	1434	WP_002920564.1	glycogen synthase GlgA	-
BWG70_RS01730	355710..358157	+	2448	WP_002920561.1	glycogen phosphorylase	-
BWG70_RS01735	358323..358670	+	348	WP_004188313.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BWG70_RS01740	358670..358969	+	300	WP_004210373.1	helix-turn-helix transcriptional regulator	Antitoxin
BWG70_RS01745	359032..360540	-	1509	WP_002920554.1	glycerol-3-phosphate dehydrogenase	-
BWG70_RS01750	360745..361074	+	330	WP_002920552.1	thiosulfate sulfurtransferase GlpE	-
BWG70_RS01755	361125..361955	+	831	WP_004151408.1	rhomboid family intramembrane serine protease GlpG	-
BWG70_RS01760	362005..362763	+	759	WP_002920548.1	DeoR/GlpR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-107)

Antitoxin

• HigA2-like, helix-turn-helix domain (9-87)

Sequences

Toxin

Protein

• Length: 116 a.a.
• Molecular weight: 13548.57 Da
• Isoelectric Point: 6.2327

>T71835 WP_004188313.1 NZ_CP019219:358323-358670 [Klebsiella pneumoniae]
MWDVETTDTFDTWFELQSRALKEDMLATMLILSEFAPQLGRPYVDTVKDSTFQNMKELRVQHHGLPIRAFFAFDPLRKAI
VLCAGNKDGMNEKRFYKEMITLADREFSQHLTKER

Nucleotide

• Length: 348 bp

>T71835 NZ_CP019219:358323-358670 [Klebsiella pneumoniae]
ATGTGGGATGTAGAAACGACGGATACGTTTGATACCTGGTTCGAATTACAAAGTAGAGCTTTAAAAGAGGATATGTTGGC
CACGATGCTCATCCTGTCTGAGTTTGCTCCGCAGCTTGGGCGCCCGTATGTTGATACGGTGAAAGATTCAACGTTTCAGA
ATATGAAAGAACTGCGGGTTCAGCATCATGGGCTCCCCATCCGCGCCTTTTTTGCTTTTGATCCTCTGCGGAAAGCTATC
GTTTTATGTGCGGGCAATAAGGATGGCATGAATGAGAAACGTTTTTACAAAGAGATGATTACTCTGGCTGACAGAGAATT
CAGCCAACACCTGACTAAGGAGCGATAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 10828.64 Da
• Isoelectric Point: 10.1464

>AT71835 WP_004210373.1 NZ_CP019219:358670-358969 [Klebsiella pneumoniae]
MVTLKELMAKQSPDSQQRIAAKAAEIRQSVALNLLREELQMSQTEMAAAMGVKQPTIAKMEQADNDPRLSTLKRYIAALG
GELSINVTLPTGKKVSFNL

Nucleotide

• Length: 300 bp

>AT71835 NZ_CP019219:358670-358969 [Klebsiella pneumoniae]
ATGGTCACGCTAAAAGAACTCATGGCGAAACAGAGTCCTGATAGCCAGCAGCGTATTGCAGCGAAAGCGGCAGAGATACG
CCAGTCTGTCGCTTTAAACTTGCTTCGAGAAGAGTTGCAAATGTCGCAAACAGAGATGGCTGCGGCTATGGGGGTAAAGC
AGCCAACGATCGCTAAAATGGAACAGGCTGATAACGACCCTCGGCTTTCGACCCTAAAACGCTACATTGCGGCATTGGGG
GGAGAACTCAGTATAAATGTCACATTGCCGACTGGAAAAAAAGTCTCGTTTAATTTGTGA

Structures

Toxin

• AlphaFold DB: A0A483JFR4