Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/Txe-RelB
Location 57428..57963 Replicon plasmid p41-1
Accession NZ_CP019209
Organism Enterococcus faecium strain 2014-VREF-41

Toxin (Protein)


Gene name yoeB Uniprot ID R2PQC0
Locus tag BVA20_RS15670 Protein ID WP_002314366.1
Coordinates 57428..57700 (-) Length 91 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID Q3Y220
Locus tag BVA20_RS15675 Protein ID WP_002297422.1
Coordinates 57700..57963 (-) Length 88 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BVA20_RS15635 52984..53627 + 644 Protein_49 transposase -
BVA20_RS15640 53703..54383 + 681 WP_002331383.1 IS6-like element IS1216 family transposase -
BVA20_RS15645 54438..54794 - 357 Protein_51 hypothetical protein -
BVA20_RS15650 55054..55602 - 549 WP_002292681.1 hypothetical protein -
BVA20_RS15655 55603..56460 - 858 WP_002292680.1 AAA family ATPase -
BVA20_RS15660 56746..57032 - 287 Protein_54 type II toxin-antitoxin system RelE/ParE family toxin -
BVA20_RS15665 57022..57351 - 330 WP_002292678.1 AbrB family transcriptional regulator -
BVA20_RS15670 57428..57700 - 273 WP_002314366.1 Txe/YoeB family addiction module toxin Toxin
BVA20_RS15675 57700..57963 - 264 WP_002297422.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
BVA20_RS15680 58197..59105 + 909 Protein_58 IS982 family transposase -
BVA20_RS15685 59108..60283 - 1176 WP_002336205.1 MFS transporter -
BVA20_RS15690 60286..62535 - 2250 WP_002314387.1 alpha-galactosidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid aac(6')-aph(2'') - 1..215998 215998
- inside IScluster/Tn - - 44004..68564 24560


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(9-87)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(5-69)


Sequences

Toxin        


Download         Length: 91 a.a.        Molecular weight: 10780.24 Da        Isoelectric Point: 9.9724

>T71830 WP_002314366.1 NZ_CP019209:c57700-57428 [Enterococcus faecium]
MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQSFEKLKPTHEGRYSRRLNRQHRVVYKVDEDEHVVEI
YSARTHYESQ

Download         Length: 273 bp

>T71830 NZ_CP019209:c57700-57428 [Enterococcus faecium]
ATGAATAACTATTCTGTGATGATTAAAAACTCGGCTAAAGCTGATTTAAAGAAAATCAAACAGTCTAATTTGAAACCTCA
ATTTGAGAAGGTTATTCAGACTTTAAAGGAAGATCCCTATCTCCCTACCCAATCATTTGAGAAGTTAAAACCTACACATG
AAGGTAGGTATTCTAGAAGATTGAATAGACAACACCGGGTTGTTTATAAGGTAGATGAAGATGAACACGTGGTTGAAATT
TATTCAGCAAGGACGCATTACGAGTCACAGTAA

Antitoxin


Download         Length: 88 a.a.        Molecular weight: 9959.22 Da        Isoelectric Point: 4.5370

>AT71830 WP_002297422.1 NZ_CP019209:c57963-57700 [Enterococcus faecium]
MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIGLDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVD
EIDWDNL

Download         Length: 264 bp

>AT71830 NZ_CP019209:c57963-57700 [Enterococcus faecium]
ATGGCAAAAACAGTCTTAAATCCTAGCTCAGCACGGAAAAATTTTTATAAGTTGTTGAAAGATGTAAATGAGAATCATAC
GGAAATTGAAATCATTAGTGAGCGCAGTGAAAATAATGCGGTGCTTATTGGTTTGGATGATTGGAAAGCAATCAAAGAAA
CGCTATTACTTGAGCAAATAGGCACTTTGGGTGTTGTTCGTAAGCGAGAAAAAGATGATAGTGGATTTACTAATGTTGAT
GAAATTGATTGGGATAACTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829F704

Antitoxin

Source ID Structure
AlphaFold DB A0A829F006

References