Detailed information of TA system TA071804

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1390644..1391315
• Replicon: chromosome
• Accession: NZ_CP019171
• Organism: MAG: Delftia acidovorans isolate ANG1

Toxin (Protein)

• Gene name: graT
• Uniprot ID: A0A543BG95
• Locus tag: AO536_RS06395
• Protein ID: WP_012203142.1
• Coordinates: 1391034..1391315 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: graA
• Uniprot ID: -
• Locus tag: AO536_RS06390
• Protein ID: WP_012203141.1
• Coordinates: 1390644..1390955 (-)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AO536_RS06380	1386144..1387157	+	1014	WP_012203140.1	type I-F CRISPR-associated endonuclease Cas1f	-
AO536_RS06385	1387154..1389736	+	2583	WP_225971044.1	type I-F CRISPR-associated helicase Cas3f	-
AO536_RS06390	1390644..1390955	-	312	WP_012203141.1	HigA family addiction module antitoxin	Antitoxin
AO536_RS06395	1391034..1391315	-	282	WP_012203142.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AO536_RS06400	1391486..1392883	+	1398	WP_012203143.1	type I-F CRISPR-associated protein Csy1	-
AO536_RS06405	1392880..1393968	+	1089	WP_012203144.1	type I-F CRISPR-associated protein Csy2	-
AO536_RS06410	1394023..1395051	+	1029	WP_012203145.1	type I-F CRISPR-associated protein Csy3	-
AO536_RS06415	1395057..1395623	+	567	WP_043783206.1	type I-F CRISPR-associated endoribonuclease Cas6/Csy4	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-93)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-98)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10679.22 Da
• Isoelectric Point: 8.5442

>T71804 WP_012203142.1 NZ_CP019171:c1391315-1391034 [Delftia acidovorans]
MAITSFKCPETQRLFTSGKTRRFANIQSVAERKLAQLDAAVTLEFLRSPPGNRLEMLVGDRQGQHSIRINDQWRVCFVWT
EKGVADVEIVDYH

Nucleotide

• Length: 282 bp

>T71804 NZ_CP019171:c1391315-1391034 [Delftia acidovorans]
ATGGCGATCACTTCCTTCAAGTGCCCGGAAACCCAGCGCCTGTTCACCAGCGGCAAGACCCGCCGGTTCGCCAACATCCA
GAGCGTGGCGGAGCGCAAGCTGGCTCAGTTGGATGCGGCTGTGACACTTGAATTTCTGCGCAGCCCTCCTGGCAATCGCC
TGGAAATGCTCGTGGGAGATCGGCAGGGCCAGCACAGCATCCGCATCAACGACCAGTGGCGCGTCTGCTTTGTCTGGACG
GAAAAGGGAGTGGCTGACGTAGAGATAGTGGATTACCACTGA

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11615.32 Da
• Isoelectric Point: 8.6927

>AT71804 WP_012203141.1 NZ_CP019171:c1390955-1390644 [Delftia acidovorans]
MLKNGMRPIHPGEVLREDYLKPMDMSANALARHLHVPASRVNDIVLERRGITADTALRLSRFFGGDAQSWLNLQTAYDLR
TAELNAELQASVRQVHPMQLHTA

Nucleotide

• Length: 312 bp

>AT71804 NZ_CP019171:c1390955-1390644 [Delftia acidovorans]
ATGCTTAAAAACGGCATGCGCCCCATACACCCAGGTGAAGTCCTGCGCGAGGACTACCTGAAGCCCATGGACATGAGCGC
CAACGCGCTGGCCAGGCACCTGCACGTTCCCGCCTCGCGCGTGAACGACATCGTGCTGGAGCGGCGCGGCATCACGGCCG
ACACGGCGCTGCGGCTGAGCCGCTTCTTTGGGGGCGACGCCCAGTCCTGGCTGAACCTTCAGACCGCCTACGACCTGCGC
ACGGCAGAGCTGAACGCGGAGTTGCAGGCGTCCGTGAGGCAAGTTCATCCGATGCAGCTGCATACCGCCTGA

Structures

Toxin

• AlphaFold DB: A0A543BG95