Detailed information of TA system TA071712

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 115093..115347
• Replicon: plasmid p1493-5
• Accession: NZ_CP019076
• Organism: Escherichia coli strain CRE1493

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: -
• Locus tag: BVL38_RS27495
• Protein ID: WP_032215358.1
• Coordinates: 115093..115299 (-)
• Length: 69 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	115286..115347 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BVL38_RS27460	111116..111517	-	402	WP_001398199.1	type II toxin-antitoxin system toxin endoribonuclease PemK	-
BVL38_RS27915	111450..111707	-	258	WP_000557619.1	type II toxin-antitoxin system antitoxin PemI	-
BVL38_RS27465	111800..112453	-	654	WP_000616807.1	CPBP family intramembrane metalloprotease	-
BVL38_RS27475	113392..114249	-	858	WP_029702152.1	incFII family plasmid replication initiator RepA	-
BVL38_RS27480	114242..114316	-	75	WP_001365705.1	RepA leader peptide Tap	-
BVL38_RS27490	114552..114809	-	258	WP_000083833.1	replication regulatory protein RepA	-
BVL38_RS27495	115093..115299	-	207	WP_032215358.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	115286..115347	+	62	NuclAT_1	-	Antitoxin
-	115286..115347	+	62	NuclAT_1	-	Antitoxin
-	115286..115347	+	62	NuclAT_1	-	Antitoxin
-	115286..115347	+	62	NuclAT_1	-	Antitoxin
BVL38_RS28140	115486..115752	-	267	WP_087757657.1	hypothetical protein	-
BVL38_RS27510	116127..116696	-	570	WP_029702150.1	DUF2726 domain-containing protein	-
BVL38_RS27515	116848..117474	-	627	WP_029702149.1	hypothetical protein	-
BVL38_RS27520	117886..118095	-	210	WP_039023209.1	hypothetical protein	-
BVL38_RS27525	118226..118786	-	561	WP_001567328.1	fertility inhibition protein FinO	-
BVL38_RS27530	118841..119587	-	747	WP_052273601.1	conjugal transfer pilus acetylase TraX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	dfrA17 / aadA5 / qacE / sul1 / mph(A) / aac(3)-IIa / blaCTX-M-15 / blaOXA-1 / aac(6')-Ib-cr / tet(A)	-	1..127772	127772

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (23-64)

Sequences

Toxin

Protein

• Length: 69 a.a.
• Molecular weight: 7778.27 Da
• Isoelectric Point: 8.8807

>T71712 WP_032215358.1 NZ_CP019076:c115299-115093 [Escherichia coli]
MKYLNTTDCSLFLAERSKFMTKYALIGVLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 207 bp

>T71712 NZ_CP019076:c115299-115093 [Escherichia coli]
ATGAAGTACCTTAACACTACTGATTGTAGCCTCTTCCTTGCAGAGAGGTCAAAGTTTATGACGAAATATGCCCTTATCGG
GGTGCTCGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTTATGTGAACTGAATATTCACAGGG
GAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Nucleotide

• Length: 62 bp

>AT71712 NZ_CP019076:115286-115347 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Antitoxin