Detailed information of TA system TA071706

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 28611..29032
• Replicon: plasmid p1493-5
• Accession: NZ_CP019076
• Organism: Escherichia coli strain CRE1493

Toxin (Protein)

• Gene name: hok
• Uniprot ID: B1VC78
• Locus tag: BVL38_RS26895
• Protein ID: WP_001302184.1
• Coordinates: 28611..28769 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	28834..29032 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BVL38_RS26860	23675..23890	-	216	WP_001352842.1	conjugal transfer relaxosome protein TraY	-
BVL38_RS26865	24026..24673	-	648	WP_000332520.1	conjugal transfer transcriptional regulator TraJ	-
BVL38_RS26870	24864..25247	-	384	WP_001063020.1	relaxosome protein TraM	-
BVL38_RS26875	25617..26189	+	573	WP_000949660.1	transglycosylase SLT domain-containing protein	-
BVL38_RS26880	26485..27306	-	822	WP_001234465.1	DUF945 domain-containing protein	-
BVL38_RS26885	27425..27712	-	288	WP_000107242.1	hypothetical protein	-
BVL38_RS28115	27737..27943	-	207	WP_000275854.1	hypothetical protein	-
BVL38_RS26895	28611..28769	-	159	WP_001302184.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	28834..29032	-	199	NuclAT_0	-	Antitoxin
-	28834..29032	-	199	NuclAT_0	-	Antitoxin
-	28834..29032	-	199	NuclAT_0	-	Antitoxin
-	28834..29032	-	199	NuclAT_0	-	Antitoxin
BVL38_RS26900	29001..29106	-	106	Protein_37	plasmid SOS inhibition protein A	-
BVL38_RS26905	29261..29578	+	318	Protein_38	IS110 family transposase	-
BVL38_RS26910	29867..30118	-	252	Protein_39	iron-containing alcohol dehydrogenase	-
BVL38_RS26915	30203..30826	-	624	WP_000912556.1	potassium channel family protein	-
BVL38_RS26920	30842..31535	-	694	Protein_41	IS1 family transposase	-
BVL38_RS26930	31592..31696	+	105	Protein_42	thiosulfate reductase cytochrome B subunit	-
BVL38_RS26940	31942..33336	-	1395	WP_001037799.1	carbohydrate porin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	dfrA17 / aadA5 / qacE / sul1 / mph(A) / aac(3)-IIa / blaCTX-M-15 / blaOXA-1 / aac(6')-Ib-cr / tet(A)	-	1..127772	127772
	-	flank	IS/Tn	-	-	30842..31219	377

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6108.35 Da
• Isoelectric Point: 9.1977

>T71706 WP_001302184.1 NZ_CP019076:c28769-28611 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGYREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T71706 NZ_CP019076:c28769-28611 [Escherichia coli]
ATGAAACTACCACGCAGCTCTCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGATACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 199 bp

>AT71706 NZ_CP019076:c29032-28834 [Escherichia coli]
TCACACAGATTACCCGTAAACAGTCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGGATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAGGA
CAAAAGCCCCGTAGTTAATTTTTCATTAACCCACGAGGC

Structures

Antitoxin