Detailed information of TA system TA071698

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3799500..3800194
• Replicon: chromosome
• Accession: NZ_CP019071
• Organism: Escherichia coli strain CRE1493

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: Q47157
• Locus tag: BVL38_RS19630
• Protein ID: WP_001263489.1
• Coordinates: 3799500..3799898 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: BVL38_RS19635
• Protein ID: WP_000554758.1
• Coordinates: 3799901..3800194 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
-	3795088..3795168	-	81	NuclAT_12	-	-
-	3795088..3795168	-	81	NuclAT_12	-	-
-	3795088..3795168	-	81	NuclAT_12	-	-
-	3795088..3795168	-	81	NuclAT_12	-	-
BVL38_RS19600	3795764..3796222	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
BVL38_RS19605	3796483..3797940	+	1458	WP_001292994.1	cytosol nonspecific dipeptidase	-
BVL38_RS19610	3797997..3798518	-	522	Protein_3574	peptide chain release factor H	-
BVL38_RS19615	3798514..3798720	-	207	Protein_3575	RtcB family protein	-
BVL38_RS19625	3799038..3799490	-	453	WP_001059892.1	GNAT family N-acetyltransferase	-
BVL38_RS19630	3799500..3799898	-	399	WP_001263489.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
BVL38_RS19635	3799901..3800194	-	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
BVL38_RS19640	3800246..3801301	-	1056	WP_001226164.1	DNA polymerase IV	-
BVL38_RS19645	3801372..3802157	-	786	WP_000207552.1	putative lateral flagellar export/assembly protein LafU	-
BVL38_RS19650	3802129..3803841	+	1713	Protein_3581	flagellar biosynthesis protein FlhA	-
BVL38_RS19655	3804065..3804562	-	498	WP_000006255.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	3721984..3805364	83380

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15487.84 Da
• Isoelectric Point: 7.4215

>T71698 WP_001263489.1 NZ_CP019071:c3799898-3799500 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T71698 NZ_CP019071:c3799898-3799500 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCCTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT71698 WP_000554758.1 NZ_CP019071:c3800194-3799901 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT71698 NZ_CP019071:c3800194-3799901 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: A0A090J8B1

Antitoxin

• AlphaFold DB: S1QAE3