Detailed information of TA system TA071654

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 407247..407968
• Replicon: chromosome
• Accession: NZ_CP019062
• Organism: Rahnella sp. ERMR1:05

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: BV494_RS01870
• Protein ID: WP_104921311.1
• Coordinates: 407657..407968 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: BV494_RS01865
• Protein ID: WP_104921310.1
• Coordinates: 407247..407660 (-)
• Length: 138 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BV494_RS01850	403086..405263	+	2178	WP_104921307.1	type I secretion system permease/ATPase	-
BV494_RS01855	405281..406450	+	1170	WP_104921308.1	HlyD family efflux transporter periplasmic adaptor subunit	-
BV494_RS01865	407247..407660	-	414	WP_104921310.1	helix-turn-helix domain-containing protein	Antitoxin
BV494_RS01870	407657..407968	-	312	WP_104921311.1	type II toxin-antitoxin system HigB family toxin	Toxin
BV494_RS01875	408066..410096	-	2031	WP_104921312.1	DUF4034 domain-containing protein	-
BV494_RS01880	410863..411984	+	1122	WP_104921313.1	porin OmpC	-
BV494_RS01885	412098..412484	-	387	WP_104921314.1	helix-turn-helix domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (20-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (82-133)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12257.11 Da
• Isoelectric Point: 10.1872

>T71654 WP_104921311.1 NZ_CP019062:c407968-407657 [Rahnella sp. ERMR1:05]
MHVIAKEAFDQAIRLHPNDKTALLATYRALKESDYPTPEHLKSVFPSLDNFRYRPKWWVINVGGNNLRLIAYINFVNKRL
YIKHIVTHGVYDQLNKHYRTYTQ

Nucleotide

• Length: 312 bp

>T71654 NZ_CP019062:c407968-407657 [Rahnella sp. ERMR1:05]
ATGCATGTTATCGCAAAGGAAGCATTCGACCAGGCTATACGGCTGCATCCAAACGACAAAACGGCTTTACTGGCAACTTA
TCGCGCGCTCAAAGAGAGTGATTATCCCACGCCAGAGCATTTGAAATCCGTATTTCCCAGTCTCGATAACTTCCGCTACA
GGCCGAAATGGTGGGTTATAAACGTCGGCGGTAATAACCTTCGCCTCATTGCCTATATCAATTTCGTGAACAAACGGTTG
TATATCAAACACATCGTTACACACGGGGTTTACGATCAATTGAACAAACACTACAGGACTTACACACAATGA

Antitoxin

Protein

• Length: 138 a.a.
• Molecular weight: 15146.41 Da
• Isoelectric Point: 4.9801

>AT71654 WP_104921310.1 NZ_CP019062:c407660-407247 [Rahnella sp. ERMR1:05]
MIAEAIEASKALMKAVPLLGGSTSTADYKSALELIDYLIEHDDENPLIEFLSAKIESYERLHADFVPFNTALENLPRGVE
ALRQLMKDYGLKQSDLREEIGGKSLVSQILSGKRSLTVEHIRALAVRFNVPTSVFIG

Nucleotide

• Length: 414 bp

>AT71654 NZ_CP019062:c407660-407247 [Rahnella sp. ERMR1:05]
ATGATCGCGGAAGCCATTGAAGCCAGCAAAGCGCTGATGAAAGCGGTGCCGTTGCTTGGCGGAAGTACCAGCACGGCCGA
TTATAAAAGTGCACTCGAGCTGATTGATTACCTGATTGAACACGATGATGAAAACCCGCTTATCGAGTTCCTGAGCGCAA
AAATTGAAAGTTACGAGCGCCTTCATGCCGATTTTGTGCCGTTCAATACCGCACTGGAAAATCTGCCCCGCGGCGTGGAA
GCCTTGCGCCAGCTGATGAAAGACTACGGACTGAAACAAAGTGATTTGCGTGAAGAGATCGGCGGTAAATCGCTGGTCAG
TCAGATACTGAGCGGTAAACGCTCGCTGACCGTGGAGCATATCCGCGCACTTGCGGTACGGTTTAATGTTCCGACAAGTG
TGTTCATCGGGTAG