Detailed information of TA system TA071649

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 174633..174887
• Replicon: plasmid p1540-4
• Accession: NZ_CP019055
• Organism: Escherichia coli strain CRE1540

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: -
• Locus tag: BVL39_RS28705
• Protein ID: WP_001351576.1
• Coordinates: 174681..174887 (+)
• Length: 69 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	174633..174694 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BVL39_RS28680	171381..172127	+	747	WP_000205749.1	conjugal transfer pilus acetylase TraX	-
BVL39_RS28685	172186..173046	+	861	WP_000704513.1	alpha/beta hydrolase	-
BVL39_RS28690	173149..173709	+	561	WP_000139315.1	fertility inhibition protein FinO	-
BVL39_RS28695	173845..174057	+	213	WP_001299730.1	ANR family transcriptional regulator	-
BVL39_RS29380	174303..174377	+	75	Protein_179	endonuclease	-
-	174633..174694	-	62	NuclAT_1	-	Antitoxin
-	174633..174694	-	62	NuclAT_1	-	Antitoxin
-	174633..174694	-	62	NuclAT_1	-	Antitoxin
-	174633..174694	-	62	NuclAT_1	-	Antitoxin
BVL39_RS28705	174681..174887	+	207	WP_001351576.1	type I toxin-antitoxin system Hok family toxin	Toxin
BVL39_RS28710	175171..175428	+	258	WP_000083845.1	replication regulatory protein RepA	-
BVL39_RS28720	175660..175734	+	75	WP_001442103.1	RepA leader peptide Tap	-
BVL39_RS28725	175727..176209	+	483	WP_001273588.1	hypothetical protein	-
BVL39_RS28730	176202..177059	+	858	WP_000774297.1	incFII family plasmid replication initiator RepA	-
BVL39_RS28735	178022..178273	+	252	WP_001132900.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
BVL39_RS28740	178270..178557	+	288	WP_000222760.1	type II toxin-antitoxin system RelE/ParE family toxin	-
BVL39_RS28750	178997..179344	-	348	Protein_187	IS1-like element IS1A family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sitABCD	vat / iroN / iroE / iroD / iroC / iroB / iucA / iucB / iucC / iucD / iutA	1..184166	184166

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (23-64)

Sequences

Toxin

Protein

• Length: 69 a.a.
• Molecular weight: 7826.31 Da
• Isoelectric Point: 8.8807

>T71649 WP_001351576.1 NZ_CP019055:174681-174887 [Escherichia coli]
MKYLNTTDCSLFFAERSKFMTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 207 bp

>T71649 NZ_CP019055:174681-174887 [Escherichia coli]
ATGAAGTACCTTAACACTACTGATTGTAGCCTCTTCTTTGCTGAGAGGTCAAAGTTTATGACGAAATATGCCCTTATCGG
GTTGCTCGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTTATGTGAGCTGAATATTCACAGAG
GAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Nucleotide

• Length: 62 bp

>AT71649 NZ_CP019055:c174694-174633 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Antitoxin