Detailed information of TA system TA071645

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 150362..150631
• Replicon: plasmid p1540-4
• Accession: NZ_CP019055
• Organism: Escherichia coli strain CRE1540

Toxin (Protein)

• Gene name: hok
• Uniprot ID: B1VC78
• Locus tag: BVL39_RS28565
• Protein ID: WP_001302184.1
• Coordinates: 150473..150631 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	150362..150427 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BVL39_RS28540	146455..146889	+	435	WP_000845962.1	conjugation system SOS inhibitor PsiB	-
BVL39_RS28545	146886..147089	+	204	Protein_148	plasmid SOS inhibition protein A	-
BVL39_RS28550	147171..147917	-	747	WP_001016257.1	IS21-like element ISEc12 family helper ATPase IstB	-
BVL39_RS28555	147932..149473	-	1542	WP_001298859.1	IS21-like element ISEc12 family transposase	-
BVL39_RS28560	149666..150236	+	571	Protein_151	plasmid SOS inhibition protein A	-
-	150205..150429	+	225	NuclAT_0	-	-
-	150205..150429	+	225	NuclAT_0	-	-
-	150205..150429	+	225	NuclAT_0	-	-
-	150205..150429	+	225	NuclAT_0	-	-
-	150362..150427	+	66	-	-	Antitoxin
BVL39_RS28565	150473..150631	+	159	WP_001302184.1	type I toxin-antitoxin system Hok family toxin	Toxin
BVL39_RS28575	151257..151553	+	297	WP_001272245.1	hypothetical protein	-
BVL39_RS28580	151664..152485	+	822	WP_001234487.1	DUF945 domain-containing protein	-
BVL39_RS28585	152782..153384	-	603	WP_077250849.1	transglycosylase SLT domain-containing protein	-
BVL39_RS28590	153705..154088	+	384	WP_001151524.1	relaxosome protein TraM	-
BVL39_RS28595	154275..154964	+	690	WP_000283385.1	conjugal transfer transcriptional regulator TraJ	-
BVL39_RS28600	155063..155458	+	396	WP_001309237.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sitABCD	vat / iroN / iroE / iroD / iroC / iroB / iucA / iucB / iucC / iucD / iutA	1..184166	184166

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6108.35 Da
• Isoelectric Point: 9.1977

>T71645 WP_001302184.1 NZ_CP019055:150473-150631 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGYREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T71645 NZ_CP019055:150473-150631 [Escherichia coli]
ATGAAACTACCACGCAGCTCTCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGATACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 66 bp

>AT71645 NZ_CP019055:150362-150427 [Escherichia coli]
AGAAGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCAC

Structures

Antitoxin