Detailed information of TA system TA071616

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 1188280..1188882
• Replicon: chromosome
• Accession: NZ_CP019051
• Organism: Escherichia coli strain CRE1540

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: BVL39_RS05910
• Protein ID: WP_000897305.1
• Coordinates: 1188571..1188882 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: BVL39_RS05905
• Protein ID: WP_000356397.1
• Coordinates: 1188280..1188570 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BVL39_RS05875	1184225..1185127	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
BVL39_RS05880	1185124..1185759	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
BVL39_RS05885	1185756..1186685	+	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
BVL39_RS05890	1187015..1187257	-	243	WP_001086388.1	hypothetical protein	-
BVL39_RS05895	1187476..1187694	-	219	WP_001611167.1	ribbon-helix-helix domain-containing protein	-
BVL39_RS05905	1188280..1188570	-	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
BVL39_RS05910	1188571..1188882	-	312	WP_000897305.1	hypothetical protein	Toxin
BVL39_RS05915	1189111..1190019	+	909	WP_001162704.1	alpha/beta hydrolase	-
BVL39_RS05920	1190083..1191024	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
BVL39_RS05925	1191069..1191506	-	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
BVL39_RS05930	1191503..1192375	-	873	WP_000920762.1	virulence factor BrkB family protein	-
BVL39_RS05935	1192369..1192968	-	600	WP_001295269.1	glucose-1-phosphatase	-
BVL39_RS05940	1193067..1193852	-	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T71616 WP_000897305.1 NZ_CP019051:c1188882-1188571 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T71616 NZ_CP019051:c1188882-1188571 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTTTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT71616 WP_000356397.1 NZ_CP019051:c1188570-1188280 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT71616 NZ_CP019051:c1188570-1188280 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7