Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 345687..346228 Replicon chromosome
Accession NZ_CP019051
Organism Escherichia coli strain CRE1540

Toxin (Protein)


Gene name yafQ Uniprot ID E9TC49
Locus tag BVL39_RS01780 Protein ID WP_000615982.1
Coordinates 345950..346228 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag BVL39_RS01775 Protein ID WP_000729704.1
Coordinates 345687..345947 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BVL39_RS01750 341470..342255 - 786 WP_000207575.1 putative lateral flagellar export/assembly protein LafU -
BVL39_RS01755 342227..343939 + 1713 Protein_317 flagellar biosynthesis protein FlhA -
BVL39_RS01760 344044..344322 + 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
BVL39_RS01765 344315..344671 + 357 WP_001030484.1 type II toxin-antitoxin system HicB family antitoxin -
BVL39_RS01770 344728..345501 - 774 WP_000543899.1 C40 family peptidase -
BVL39_RS01775 345687..345947 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
BVL39_RS01780 345950..346228 + 279 WP_000615982.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
BVL39_RS01785 346384..347124 + 741 WP_001225679.1 murein L,D-transpeptidase -
BVL39_RS01790 347095..347862 - 768 WP_000333380.1 class II glutamine amidotransferase -
BVL39_RS01795 348068..348646 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10816.59 Da        Isoelectric Point: 10.0702

>T71613 WP_000615982.1 NZ_CP019051:345950-346228 [Escherichia coli]
MIQRDIEYSGQFSKDVKVAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T71613 NZ_CP019051:345950-346228 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAAGTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT71613 WP_000729704.1 NZ_CP019051:345687-345947 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT71613 NZ_CP019051:345687-345947 [Escherichia coli]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References