Detailed information of TA system TA071609

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 56023..56750
• Replicon: plasmid pRJA166b
• Accession: NZ_CP019049
• Organism: Klebsiella pneumoniae subsp. pneumoniae strain RJA166

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7W3D9W1
• Locus tag: RJA_RS28070
• Protein ID: WP_011251285.1
• Coordinates: 56023..56334 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: RJA_RS28075
• Protein ID: WP_011251286.1
• Coordinates: 56331..56750 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RJA_RS28030	51084..51599	-	516	WP_041937820.1	hypothetical protein	-
RJA_RS28035	51707..52282	-	576	WP_011251279.1	hypothetical protein	-
RJA_RS28040	52399..53355	-	957	WP_011251280.1	DsbA family protein	-
RJA_RS28045	53415..53756	-	342	WP_011251281.1	hypothetical protein	-
RJA_RS28050	53770..54081	-	312	WP_011251282.1	hypothetical protein	-
RJA_RS28055	54098..54532	-	435	Protein_62	thioredoxin fold domain-containing protein	-
RJA_RS28060	54549..55246	-	698	Protein_63	IS1 family transposase	-
RJA_RS28065	55360..55818	+	459	Protein_64	DDE-type integrase/transposase/recombinase	-
RJA_RS28070	56023..56334	+	312	WP_011251285.1	type II toxin-antitoxin system HigB family toxin	Toxin
RJA_RS28075	56331..56750	+	420	WP_011251286.1	helix-turn-helix domain-containing protein	Antitoxin
RJA_RS28080	56897..57865	+	969	WP_074428168.1	IS5 family transposase	-
RJA_RS28085	57967..58302	-	336	Protein_68	conjugal transfer protein TraI	-
RJA_RS28090	58316..59104	-	789	WP_040217257.1	hypothetical protein	-
RJA_RS28095	59125..59568	-	444	WP_048333569.1	hypothetical protein	-
RJA_RS30580	60164..60919	+	756	Protein_71	hypothetical protein	-
RJA_RS28110	61112..61582	+	471	WP_048333570.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	iroB / rmpA / iutA / iucD / iucC / iucB / iucA / rmpA / iroN / iroD / iroC	1..228613	228613
	-	inside	IScluster/Tn	-	-	54549..66871	12322

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12346.12 Da
• Isoelectric Point: 9.7248

>T71609 WP_011251285.1 NZ_CP019049:56023-56334 [Klebsiella pneumoniae subsp. pneumoniae]
MHVISKEPFEEAAKRYPNDSLAIRALYRLVRETDFSSPAEMLTLIPSLDNFKYRNKWWVLDVGGNNLRVIAYINFVNKRF
YVKHIATHADYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T71609 NZ_CP019049:56023-56334 [Klebsiella pneumoniae subsp. pneumoniae]
ATGCACGTAATTTCAAAAGAGCCTTTTGAGGAAGCGGCAAAGCGATATCCCAACGATTCGTTAGCCATTCGGGCGTTGTA
CCGCTTAGTGCGCGAGACGGACTTCTCTTCTCCAGCTGAGATGCTAACTCTGATACCGAGCCTGGATAATTTTAAGTACA
GGAATAAGTGGTGGGTTTTGGATGTAGGGGGCAACAACTTAAGGGTTATCGCCTATATCAATTTCGTAAATAAGCGCTTC
TACGTGAAGCACATCGCAACCCACGCTGATTACGACAAGTTGACCCGCTATTACAGGGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15377.46 Da
• Isoelectric Point: 4.4420

>AT71609 WP_011251286.1 NZ_CP019049:56331-56750 [Klebsiella pneumoniae subsp. pneumoniae]
MITDTAKAIEATKQLVAAVPFLGGSSSESDYREAMELVDYLIENDDENPLIDFLASKIADYEDNSPRFAEFNKAIAEIPV
GVALLRTLIDQHKLSYSDLKDEIGSKSLVSQILSGQRSLTITHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT71609 NZ_CP019049:56331-56750 [Klebsiella pneumoniae subsp. pneumoniae]
ATGATTACCGACACTGCAAAAGCCATTGAAGCAACCAAACAACTCGTAGCTGCCGTCCCCTTCCTAGGGGGCAGTTCTTC
CGAGAGCGATTACCGCGAAGCGATGGAGCTGGTGGATTATCTGATCGAGAACGACGACGAGAACCCACTTATTGACTTCC
TGGCGAGTAAGATTGCCGACTATGAAGATAACAGCCCTCGTTTCGCTGAGTTCAACAAAGCTATTGCTGAAATACCAGTC
GGCGTTGCACTTCTGCGAACCCTGATTGACCAGCACAAGCTGTCTTATTCAGACCTTAAGGATGAGATCGGATCTAAATC
ATTGGTCAGCCAAATCCTGTCAGGGCAGCGCTCTTTGACCATCACTCACATAAAAGCTCTTTCCGCGCGCTTCGGTGTAA
AACCTGAGTGGTTCCTATAA

Structures

Toxin

• AlphaFold DB: A0A7W3D9W1